Description

Ergocalciferol, Vit D2-D6 CAS NO 1311259-89-8 is a high-purity, deuterium-labeled analog of Vitamin D2 (Ergocalciferol), a critical secosteroid molecule. This stable isotope-labeled compound is essential for advanced analytical and research applications where precise quantification and metabolic pathway tracing are required. It is primarily utilized by pharmaceutical R&D laboratories, clinical research organizations, and analytical reference standard manufacturers for applications in mass spectrometry, pharmacokinetic studies, and internal standard methodologies.

Application

• Internal Standard for LC-MS/MS Analysis: Serves as a critical quantitative reference in the accurate measurement of Vitamin D2 levels in complex biological matrices like serum, plasma, and tissues.
• Drug Metabolism and Pharmacokinetics (DMPK) Studies: Used to trace and quantify the absorption, distribution, metabolism, and excretion (ADME) of ergocalciferol in preclinical and clinical research.
• Clinical Diagnostics Development: Employed in the development and validation of high-sensitivity assays for Vitamin D status assessment and related endocrine disorders.
• Nutritional Research: Facilitates precise studies on the bioavailability, bioequivalence, and metabolic fate of Vitamin D2 from fortified foods and supplements.
• Pharmaceutical Quality Control: Acts as a reference standard in the purity and potency testing of pharmaceutical formulations containing Vitamin D2.
• Biochemical Research: Used in mechanistic studies to elucidate the role of Vitamin D2 and its metabolites in cellular processes and signaling pathways.

Basic Information

Product Name	Ergocalciferol, Vit D2-D6
CAS No.	1311259-89-8
Molecular Formula	C₂₈H₃₈D₆O
Molecular Weight	~402.66 g/mol
Synonyms	Vitamin D2-d6; [6,19,19-²H₆]Ergocalciferol; Deuterated Vitamin D2; Ergocalciferol-d6; 9,10-Seco(5Z,7E,22E)-5,7,10(19),22-ergostatetraen-3-ol-d6; (3β,5Z,7E,22E)-9,10-Secoergosta-5,7,10(19),22-tetraen-3-ol-d6; Stable Isotope Labeled Vitamin D2
EINECS	Contact for details

Quality Control

Our Ergocalciferol-d6 is manufactured under strict quality systems to ensure the highest isotopic and chemical purity required for sensitive analytical applications. Each batch is characterized by advanced spectroscopic and chromatographic techniques, including NMR, HPLC, and mass spectrometry, to confirm identity, isotopic enrichment, and purity. A comprehensive Certificate of Analysis (COA) detailing batch-specific results for assay, isotopic purity, and related substances is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) to minimize oxidation. For long-term storage, keep desiccated to prevent moisture absorption. Allow the sealed container to reach room temperature before opening to avoid condensation.

Specification

Item	Specification
Appearance	White to off-white crystalline solid
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to expected mass spectrum
Purity (HPLC)	≥ 98.0%
Isotopic Purity (²H₆)	≥ 98.0 atom % D
Chemical Purity (NMR)	Conforms to structure
Loss on Drying	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.