Description

L-Prolinamide-2,5,5-D3 is a high-purity, isotopically labeled derivative of the amino acid proline, specifically deuterated at the 2, 5, and 5 positions. This compound is essential for advanced research and development, providing a stable, non-radioactive tracer for precise metabolic and pharmacokinetic studies. It is primarily utilized by pharmaceutical researchers, analytical chemists, and contract research organizations (CROs) engaged in drug discovery, proteomics, and quantitative mass spectrometry.

Application

• Internal Standard for LC-MS/MS: Serves as a critical quantitative reference standard in bioanalytical method development for proline and proline-containing compounds.
• Metabolic Pathway Tracing: Used in deuterium labeling studies to investigate collagen synthesis, cellular metabolism, and amino acid turnover.
• Pharmaceutical Impurity Profiling: Aids in the identification and quantification of process-related impurities and degradation products in active pharmaceutical ingredients (APIs).
• Proteomics Research: Employed in stable isotope labeling by amino acids in cell culture (SILAC) and other quantitative proteomics techniques.
• NMR Spectroscopy: Acts as a deuterated probe to simplify complex NMR spectra and study molecular structure and dynamics.
• Precursor for Labeled Peptides: Used in the synthesis of isotopically labeled peptides for research and diagnostic applications.

Basic Information

Product Name	L-Prolinamide-2,5,5-D3
CAS No.	1276197-55-7
Molecular Formula	C5H7D3N2O
Molecular Weight	117.16 g/mol
Synonyms	(2S)-Pyrrolidine-2-carboxamide-2,5,5-D3; L-Proline amide-2,5,5-D3; Prolinamide-2,5,5-D3; (S)-2-Carbamoylpyrrolidine-2,5,5-D3; 2-Pyrrolidinecarboxamide, (2S)-, 2,5,5-D3-; Deuterated L-Prolinamide; L-Pro-NH2-2,5,5-D3
EINECS	Contact for details

Quality Control

Every batch of L-Prolinamide-2,5,5-D3 is manufactured under strict quality management protocols. We provide comprehensive analytical data to ensure identity, purity, and isotopic enrichment meet the exacting standards required for research and development. A detailed Certificate of Analysis (COA) including results for assay, chiral purity, deuterium enrichment (D%), and impurity profile is supplied with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. Due to its hygroscopic (moisture-sensitive) nature, the container should be sealed under an inert atmosphere after each use and allowed to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Assay (HPLC)	≥98.0%
Chiral Purity (HPLC)	≥99.0% (L-isomer)
Deuterium Enrichment (MS)	≥98 atom % D
Water Content (KF)	≤1.0%
Residue on Ignition	≤0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.