Description

Pentamidine-D4 2HCl (Pentane-1,1,5,5-D4) is a deuterium-labeled analog of the antiprotozoal drug pentamidine isethionate, where four hydrogen atoms are replaced with deuterium at the 1 and 5 positions of the pentane linker. This stable isotope-labeled compound is essential for use as an internal standard in quantitative bioanalytical methods, ensuring the highest accuracy and reliability in pharmacokinetic and metabolic studies. It is a critical tool for pharmaceutical R&D, contract research organizations (CROs), and analytical laboratories focused on drug development and therapeutic monitoring for diseases like Pneumocystis pneumonia and leishmaniasis.

Application

• Primary use as a stable isotope-labeled internal standard for the precise quantification of pentamidine in biological matrices (plasma, serum, tissue) via LC-MS/MS.
• Facilitates drug metabolism and pharmacokinetics (DMPK) studies, enabling accurate tracking of the parent drug's absorption, distribution, metabolism, and excretion (ADME).
• Critical for clinical research and therapeutic drug monitoring (TDM) to optimize dosing regimens and ensure patient safety.
• Supports pharmaceutical quality control and impurity profiling in the manufacturing of pentamidine-based APIs.
• Enables forensic and anti-doping analysis where precise identification and quantification of the drug are required.
• Used in academic and institutional research investigating the mechanism of action and resistance pathways of antimicrobial agents.

Basic Information

Product Name	Pentamidine-D4 2HCl (Pentane-1,1,5,5-D4)
CAS No.	1276197-32-0
Molecular Formula	C19H20D4N4O2 • 2HCl
Molecular Weight	~ 594.5 g/mol
Synonyms	1,5-Bis(4-amidinophenoxy)pentane-1,1,5,5-d4 dihydrochloride; 4,4'-(Pentane-1,1,5,5-d4-diyl)dioxy)dibenzamidine dihydrochloride; Deuterated Pentamidine Dihydrochloride; Pentamidine-d4 Dihydrochloride; [1,1,5,5-2H4]-Pentamidine 2HCl; Pentamidine (Pentane-d4) 2HCl; Pentamidine Internal Standard (D4); Pentamidine-d4 Isotopologue
EINECS	Contact for details

Quality Control

Every batch of Pentamidine-D4 2HCl is manufactured and analyzed under strict quality systems to ensure it meets the exacting standards required for analytical internal standards. We provide comprehensive Certificates of Analysis (COA) that detail critical parameters including isotopic purity (chemical and isotopic purity ≥98%), assay, and impurity profiles. Our quality commitment ensures reliable and reproducible performance in your sensitive LC-MS/MS applications, supporting compliance with GLP and GMP guidelines where applicable.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) or as specified on the label or COA. This compound is hygroscopic (moisture-sensitive) and must be kept in a dry environment. For long-term storage, consider desiccated conditions.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to expected mass spectrum
Purity (HPLC)	≥ 98.0%
Isotopic Purity	≥ 98.0% (D4)
Water Content (KF)	≤ 2.0%
Residue on Ignition	≤ 0.5%
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.