Description

Calcifediol-D3 CAS NO 1262843-45-7 is a deuterated analog of calcifediol (25-hydroxyvitamin D3), a key intermediate in the vitamin D metabolic pathway. This high-purity, isotopically labeled compound is essential for research and development in pharmaceutical and analytical chemistry, serving as a critical internal standard for mass spectrometry. It is primarily utilized by pharmaceutical R&D teams, analytical laboratories, and manufacturers of diagnostic kits who require precise quantification of vitamin D metabolites.

Application

• Pharmaceutical Reference Standard: Serves as a certified reference material (CRM) for the quantification of endogenous 25-hydroxyvitamin D3 in clinical and research assays.
• Mass Spectrometry Internal Standard: Essential for Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) methods, improving accuracy and precision in vitamin D metabolite analysis.
• Clinical Diagnostics: Used in the development and calibration of immunoassays and LC-MS/MS kits for measuring vitamin D status.
• Metabolic Pathway Research: A vital tool for studying the pharmacokinetics, metabolism, and biological activity of vitamin D3 and its derivatives.
• Drug Development: Employed in the research and quality control of new vitamin D-based therapeutics, including those for osteoporosis, psoriasis, and hyperparathyroidism.
• Forensic & Anti-Doping Analysis: Used in specialized laboratories to detect and quantify vitamin D and related steroid hormones.

Basic Information

Product Name	Calcifediol-D3
CAS No.	1262843-45-7
Molecular Formula	C27H43D3O2
Molecular Weight	401.66 g/mol (for C27H43D3O2)
Synonyms	25-Hydroxyvitamin D3-d3; 25-Hydroxycholecalciferol-d3; 25-OH-D3-d3; (3β,5Z,7E)-9,10-Secocholesta-5,7,10(19)-triene-3,25-diol-d3; 25-Hydroxy Vitamin D3 Deuterated; 25-Hydroxy VD3-d3; 25(OH)D3-d3
EINECS	Contact for details

Quality Control

Our Calcifediol-D3 is manufactured under strict quality systems to ensure the high purity and isotopic enrichment required for analytical applications. Each batch is supported by a comprehensive Certificate of Analysis (COA) detailing purity, isotopic purity (D-enrichment), and identity confirmation via advanced spectroscopic methods. We adhere to relevant guidelines for reference standard materials to ensure reliable and reproducible performance in your critical assays.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere. This product is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white crystalline solid
Identification (HPLC-MS)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 98.0%
Isotopic Purity (D-enrichment)	≥ 98 atom % D
Chemical Purity	≥ 95%
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.