Description

Metronidazole-D4 is a stable isotope-labeled analog of the widely used nitroimidazole antibiotic and antiprotozoal agent, Metronidazole. This deuterated compound is an essential internal standard for the precise and accurate quantification of Metronidazole in complex biological matrices using advanced analytical techniques like LC-MS/MS. It is primarily required by pharmaceutical R&D laboratories, contract research organizations (CROs), and analytical testing facilities engaged in pharmacokinetic studies, drug metabolism research, and bioanalytical method development and validation.

Application

• Bioanalytical Internal Standard: Serves as a critical internal standard for the quantification of Metronidazole in plasma, serum, urine, and tissue samples via mass spectrometry.
• Pharmacokinetic & Metabolism Studies: Enables precise tracking of drug absorption, distribution, metabolism, and excretion (ADME) in preclinical and clinical research.
• Method Development & Validation: Used as a reference material to develop, optimize, and validate sensitive and specific LC-MS/MS methods in compliance with GLP and FDA/EMA guidelines.
• Doping Control & Forensic Analysis: Supports accurate detection and confirmation of Metronidazole in sports doping control and forensic toxicology investigations.
• Pharmaceutical Quality Control: Can be utilized in advanced impurity profiling and stability-indicating assay methods for pharmaceutical formulations.
• Isotope Dilution Mass Spectrometry (IDMS): Employed in high-accuracy IDMS protocols for definitive measurement of Metronidazole concentrations.

Basic Information

Item	Detail
Product Name	Metronidazole-D4
CAS No.	1261392-47-5
Molecular Formula	C6H5D4N3O3
Molecular Weight	172.16 g/mol (approx.)
Synonyms	2-Methyl-5-nitro-1H-imidazole-1-ethanol-d4; 1-(2-Hydroxyethyl)-2-methyl-5-nitroimidazole-d4; Deuterated Metronidazole; Metronidazole (phenyl-D4); Metronidazole-d4; Flagyl-d4; Clont-d4; [²H₄]-Metronidazole
EINECS	Contact for details

Quality Control

Our Metronidazole-D4 is produced under strict quality-controlled conditions to ensure the high chemical and isotopic purity required for sensitive analytical applications. Each batch is fully characterized by NMR, HPLC, and mass spectrometry. A comprehensive Certificate of Analysis (COA) is provided, detailing isotopic enrichment (typically ≥98% D), chemical purity (by HPLC), and confirming structural identity. We support compliance with GMP/GLP principles for research use.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) in a dry environment. The product is light-sensitive and should be stored away from light.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity	≥ 98 atom % D
Chemical Purity	≥ 95.0%
Water Content (KF)	≤ 0.5%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.