Description

Pyridoxal-D5 5'-Phosphate is a deuterated, isotopically labeled form of the biologically active coenzyme pyridoxal 5'-phosphate (PLP). This stable isotope-labeled compound is essential for advanced analytical research, providing a critical internal standard for precise quantification in mass spectrometry-based methods. It is primarily required by pharmaceutical R&D laboratories, clinical research organizations, and academic institutions engaged in metabolomics, vitamin B6 pathway analysis, and drug metabolism studies.

Application

• Internal Standard for LC-MS/MS: Serves as a critical quantitative reference standard in liquid chromatography-tandem mass spectrometry for the analysis of endogenous vitamin B6 vitamers.
• Metabolomics Research: Used in stable isotope dilution assays to accurately measure pyridoxal phosphate levels in complex biological matrices like plasma, serum, and tissue homogenates.
• Drug Metabolism and Pharmacokinetics (DMPK): Employed in tracing and quantifying the metabolic fate of pharmaceuticals that interact with or are processed by PLP-dependent enzymes.
• Clinical Diagnostics Development: Facilitates the development of highly sensitive and specific clinical assays for assessing vitamin B6 status and related deficiencies.
• Biochemical Pathway Analysis: Enables precise tracking and flux analysis in studies of amino acid metabolism, neurotransmitter synthesis, and one-carbon metabolism where PLP is a cofactor.
• Nutritional Science Research: Provides a gold-standard tool for the accurate assessment of vitamin B6 bioavailability and metabolism in nutritional studies.

Basic Information

Product Name	Pyridoxal-D5 5'-Phosphate
CAS No.	1246818-16-5
Molecular Formula	C8H5D5NO6P
Molecular Weight	252.18 g/mol (for monohydrate, typical)
Synonyms	Pyridoxal-d5 5-Phosphate; Pyridoxal Phosphate-d5; PLP-d5; 3-Hydroxy-5-(hydroxymethyl)-2-methylisonicotinaldehyde-d5 5-Phosphate; Vitamin B6 Phosphate-d5; Deuterated Pyridoxal Phosphate; [2,4,5,6-d5]-Pyridoxal 5'-Phosphate; 4-Pyridoxic Acid 5'-Phosphate Precursor-d5
EINECS	Contact for details

Quality Control

Our Pyridoxal-D5 5'-Phosphate is manufactured under strict quality systems to ensure the highest isotopic purity and chemical integrity required for analytical applications. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing parameters such as isotopic purity (Atom % D), chemical purity (by HPLC), and confirmation of structure. We support research and development that demands reliable and traceable reference materials.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, store under inert atmosphere. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms
Isotopic Purity (Atom % D)	≥ 98% (by MS)
Chemical Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Specific Optical Rotation	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.