Description

Tizoxanide-D4 CAS NO 1246817-56-0 is a deuterated isotopologue of the antiprotozoal agent tizoxanide, specifically labeled with four deuterium atoms. This stable isotope-labeled compound is of critical importance for use as an internal standard in quantitative bioanalytical methods, ensuring the highest accuracy and reliability in pharmacokinetic and metabolic studies. It is an essential tool for pharmaceutical R&D laboratories, contract research organizations (CROs), and analytical service providers focused on developing and validating LC-MS/MS assays for the parent drug and its metabolites.

Application

• Internal Standard for LC-MS/MS Analysis: Primary application as a deuterated internal standard for the precise quantification of tizoxanide and its metabolites in biological matrices (plasma, serum, urine).
• Drug Metabolism and Pharmacokinetics (DMPK) Studies: Enables accurate tracking of drug absorption, distribution, metabolism, and excretion (ADME) in preclinical and clinical research.
• Bioanalytical Method Development & Validation: Critical for developing robust, sensitive, and specific analytical methods compliant with FDA, EMA, and ICH guidelines.
• Metabolite Identification and Profiling: Aids in the structural elucidation and quantification of drug metabolites using mass spectrometry.
• Quality Control in Pharmaceutical Manufacturing: Can be used in impurity profiling and stability-indicating assays for drug substance and product.
• Toxicology and Preclinical Safety Assessment: Supports studies investigating the safety profile and toxicokinetics of tizoxanide-based therapeutics.

Basic Information

Product Name	Tizoxanide-D4
CAS No.	1246817-56-0
Molecular Formula	C8H3D4N3O3S
Molecular Weight	226.25 g/mol (approximate)
Synonyms	2-Hydroxy-N-(5-nitro-2-thiazolyl)benzamide-d4; 2-Hydroxy-N-(5-nitro-1,3-thiazol-2-yl)benzamide-d4; Tizoxanide (phenyl-d4); Nitazoxanide Metabolite-d4; NTZ Metabolite-d4; Deuterated Tizoxanide; [2H4]-Tizoxanide
EINECS	Contact for details

Quality Control

Our Tizoxanide-D4 is manufactured under strict quality control protocols to ensure the high chemical purity and isotopic enrichment required for sensitive analytical applications. Each batch is supported by a comprehensive Certificate of Analysis (COA) detailing parameters such as isotopic purity (D-enrichment), chemical purity (by HPLC), and identity confirmation (by NMR and MS). We adhere to relevant industry standards for stable isotope-labeled compounds to guarantee reliable performance as an internal standard.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) in a dry environment. For long-term storage, consider storing desiccated at -20°C.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (HPLC-MS)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥98.0%
Isotopic Purity (D-enrichment)	≥98 atom % D
Water Content (KF)	≤0.5%
Residual Solvents (GC)	Complies with ICH Q3C

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.