Description

(2S,4R)-2-[(Dimethylamino)Carbonyl]-4-Hydroxy-1-Pyrrolidinecarboxylic Acid-D6 4-Nitrobenzyl Ester is a high-purity, deuterium-labeled derivative of a key pyrrolidinecarboxylic acid scaffold. This compound is essential for advanced analytical and research applications where isotopic labeling is required for precise tracking and quantification. It is primarily utilized by pharmaceutical researchers, analytical chemists, and contract research organizations (CROs) engaged in method development, metabolism studies, and the synthesis of isotopically labeled standards.

Application

• Internal Standard for LC-MS/MS: Serves as a critical, isotopically labeled internal standard in quantitative bioanalytical methods for pharmacokinetic and metabolomic studies.
• Metabolic Pathway Tracing: Used in drug metabolism and disposition (DMPK) research to elucidate metabolic pathways and identify metabolites using mass spectrometry.
• Pharmaceutical Reference Standard: Acts as a certified reference material (CRM) for the calibration and validation of analytical instruments in quality control laboratories.
• Protease Inhibitor Research: The pyrrolidine core is a common motif in protease inhibitor design; this labeled compound aids in the study of binding kinetics and enzyme mechanisms.
• Stable Isotope Labeling (SIL): Employed in the synthesis of more complex deuterated molecules for use as probes in chemical and biological research.
• Method Development and Validation: Provides a reliable standard for developing and validating sensitive and specific chromatographic assays in compliance with GLP/GMP guidelines.

Basic Information

Product Name	(2S,4R)-2-[(Dimethylamino)Carbonyl]-4-Hydroxy-1-Pyrrolidinecarboxylic Acid-D6 4-Nitrobenzyl Ester
CAS No.	1246814-87-8
Molecular Formula	C15H13D6N3O6 *
Molecular Weight	Contact for details
Synonyms	4-Nitrobenzyl (2S,4R)-2-[(Dimethylamino)carbonyl]-4-hydroxypyrrolidine-1-carboxylate-d6; (2S,4R)-1-[(4-Nitrobenzyl)oxycarbonyl]-4-hydroxy-2-(dimethylcarbamoyl)pyrrolidine-d6; (2S,4R)-N,N-Dimethyl-4-hydroxy-1-[(4-nitrobenzyl)oxycarbonyl]pyrrolidine-2-carboxamide-d6; Deuterated Pyrrolidinecarboxylic Acid 4-Nitrobenzyl Ester; D6-Labeled Pyrrolidine Amide Ester; CAS 1246814-87-8; PNZ-Protected Hydroxyproline Derivative-d6
EINECS	Contact for details

* Formula accounts for deuterium (D) substitution at six positions.

Quality Control

Our deuterated compounds are produced under strict quality management systems to ensure batch-to-batch consistency and traceability. Each lot undergoes comprehensive analysis including HPLC for chemical purity, NMR and MS for structural confirmation and isotopic enrichment, and residual solvent analysis. A detailed Certificate of Analysis (COA) is provided with each shipment, documenting the exact specifications and analytical results. We support compliance with cGMP and ICH Q7 guidelines for advanced pharmaceutical intermediates.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) or as specified on the label or COA. Keep the container in a dry, cool, and well-ventilated area. For long-term storage, consider storing under inert atmosphere to ensure maximum stability.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment (MS)	≥ 98 atom % D
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Complies with ICH limits

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.