Description

(2,2,3,4,4-D5)-Zymosterol is a deuterated isotopologue of the key cholesterol biosynthesis intermediate, zymosterol. This stable isotope-labeled compound is essential for advanced analytical research, enabling precise metabolic tracing and quantification in complex biological systems. It is primarily utilized by pharmaceutical R&D laboratories, academic research institutions, and CROs specializing in lipid metabolism, drug discovery, and biomarker analysis.

Application

• Internal Standard for LC-MS/MS Analysis: Critical for the accurate quantification of endogenous zymosterol and related sterols in biological matrices like plasma, serum, and tissue homogenates.
• Metabolic Pathway Tracing: Used in isotope dilution studies to elucidate the cholesterol biosynthesis pathway, enzyme kinetics, and metabolic flux in cell cultures or model organisms.
• Drug Development & Pharmacokinetics: Supports ADME (Absorption, Distribution, Metabolism, Excretion) studies for novel therapeutics targeting lipid metabolism or cholesterol homeostasis.
• Biomarker Research: Facilitates the development and validation of sensitive assays for diagnosing inborn errors of metabolism (e.g., Smith-Lemli-Opitz syndrome) or other sterol disorders.
• Stable Isotope Labeling in Chemical Synthesis: Serves as a labeled building block for the synthesis of more complex deuterated sterol probes.

Basic Information

Product Name	(2,2,3,4,4-D5)-Zymosterol
CAS No.	1246298-29-2
Molecular Formula	C27H39D5O
Molecular Weight	389.66 g/mol (approx.)
Synonyms	5α-Cholesta-8,24-dien-3β-ol-2,2,3,4,4-d5; [D5]-Zymosterol; Deuterated Zymosterol; Zymosterol-d5; 8,24-Cholestadien-3β-ol-d5; (3β)-Cholesta-8,24-dien-3-ol-d5; 5α-Cholesta-8,24-dien-3β-ol (deuterated)
EINECS	Contact for details

Quality Control

Our (2,2,3,4,4-D5)-Zymosterol is produced under strict quality-controlled conditions to ensure high chemical purity and isotopic enrichment, meeting the exacting standards of analytical and research applications. Each batch is supported by a comprehensive Certificate of Analysis (COA) detailing parameters such as chemical purity (by HPLC), isotopic purity (by MS), and identity confirmation (by NMR and/or IR). We adhere to relevant quality management frameworks to ensure supply reliability and data integrity for your research.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere for long-term stability. This compound is hygroscopic (moisture-sensitive) and light-sensitive; allow the sealed vial to equilibrate to room temperature before opening to minimize moisture uptake. For short-term use, store desiccated at 2-8°C.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (NMR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Isotopic Purity (MS)	≥ 98 atom % D
Chemical Purity (Titration)	Contact for details
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.