Description

Leukotriene E4-D5 is a stable isotope-labeled analog of the cysteinyl leukotriene Leukotriene E4 (LTE4), specifically deuterated at five positions. This compound is an essential internal standard in quantitative bioanalytical methods, providing unmatched accuracy for mass spectrometry-based assays. It is primarily utilized by pharmaceutical researchers, clinical diagnostic laboratories, and contract research organizations (CROs) engaged in biomarker discovery, inflammation research, and drug development studies.

Application

• Quantitative Mass Spectrometry Internal Standard: Serves as a critical reference compound for the precise quantification of endogenous Leukotriene E4 in complex biological matrices like urine, plasma, and tissue homogenates.
• Biomarker Analysis and Validation: Enables accurate measurement of LTE4 as a key biomarker for asthma, allergic inflammation, and other leukotriene-mediated diseases in clinical and preclinical studies.
• Drug Metabolism and Pharmacokinetics (DMPK): Used in stable isotope dilution assays to study the pharmacokinetic profile and metabolic fate of drugs targeting the leukotriene pathway.
• Eicosanoid Profiling Research: A vital component in targeted metabolomics panels for comprehensive analysis of eicosanoid and oxylipin pathways in inflammation and immunology research.
• Method Development and GLP Compliance: Essential for developing, validating, and conducting Good Laboratory Practice (GLP)-compliant bioanalytical methods requiring high precision and accuracy.
• Quality Control in Diagnostic Kit Manufacturing: Employed as a calibrant or control in the production of in-vitro diagnostic (IVD) kits for monitoring inflammatory diseases.

Basic Information

Product Name	Leukotriene E4-D5
CAS No.	1240398-14-4
Molecular Formula	C23H32D5NO7S
Molecular Weight	470.61 g/mol (approximate)
Synonyms	LTE4-d5; Deuterated Leukotriene E4; (5S,6R)-7-[(1R,2E,4E,6Z,9Z)-1-[(2S)-2-Amino-2-carboxyethyl]sulfanyl-2,4,6,9-heptadecatetraen-1-ylidene]-5,6-dihydroxy-5,6,7,8,9-pentadeuteriocyclohepta[b]pyran-4-one; 5(S),6(R)-LTE4-d5; 11-trans-LTE4-d5; [²H₅]-LTE4; LTE4 Internal Standard
EINECS	Contact for details

Quality Control

Our Leukotriene E4-D5 is manufactured under strict quality controls to ensure the high chemical and isotopic purity required for sensitive analytical applications. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity (by HPLC), isotopic enrichment (by MS), and identity confirmation. We adhere to relevant industry standards for research chemicals, and custom synthesis to specific purity or isotopic enrichment specifications is available upon request.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. Allow the sealed vial to equilibrate to room temperature before opening to minimize condensation and exposure to moisture. The product is light-sensitive (store away from light) and solutions should be prepared fresh or stored under inert atmosphere when possible due to sensitivity.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC-MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Isotopic Enrichment (MS)	≥ 98 atom % D
Chemical Purity (NMR)	Conforms
Solubility	Soluble in ethanol, methanol, DMSO; slightly soluble in aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.