Description

4-Maleimidobutyric Acid Hydrazide Trifluoroacetate is a high-purity, heterobifunctional crosslinking reagent designed for advanced bioconjugation and protein modification applications. Its core value lies in the maleimide group's specificity for thiols (cysteine residues) and the hydrazide moiety's reactivity with aldehydes and ketones, enabling the creation of stable, site-specific linkages under mild conditions. This compound is essential for researchers and manufacturers in pharmaceutical development, antibody-drug conjugate (ADC) synthesis, diagnostic assay development, and materials science, where precise and stable biomolecule attachment is critical.

Application

• Antibody-Drug Conjugate (ADC) Linker Chemistry: Site-specific conjugation of cytotoxic payloads to monoclonal antibodies via cysteine residues.
• Protein and Peptide Labeling: Introduction of fluorophores, biotin, or other probes for imaging, detection, and pull-down assays.
• Surface Immobilization: Functionalization of biosensor chips, microarrays, and nanoparticles with proteins or antibodies for diagnostic platforms.
• Polymer and Biomaterial Modification: Crosslinking agent for creating hydrogels or functional polymers with tailored biological activity.
• Enzyme Stabilization and Modification: Crosslinking enzymes to carriers or other proteins to enhance stability and reusability.
• Oligonucleotide Conjugation: Attachment of proteins or other molecules to nucleic acids for therapeutic or diagnostic constructs.

Basic Information

Product Name	4-Maleimidobutyric Acid Hydrazide Trifluoroacetate
CAS No.	1239587-68-8
Molecular Formula	C9H10F3N3O5
Molecular Weight	297.19 g/mol
Synonyms	4-Maleimidobutyric Hydrazide Trifluoroacetate; Maleimidobutyric Hydrazide TFA Salt; 4-(2,5-Dioxo-2,5-dihydro-1H-pyrrol-1-yl)butanehydrazide Trifluoroacetate; MBH-TFA; GMBS-Hydrazide•TFA; N-(3-Maleimidopropionyl)hydrazide Trifluoroacetate; 1-(3-Hydrazinyl-3-oxopropyl)-1H-pyrrole-2,5-dione Trifluoroacetate
EINECS	Contact for details

Quality Control

Our 4-Maleimidobutyric Acid Hydrazide Trifluoroacetate is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry, to ensure it meets the high standards required for sensitive bioconjugation work. Certificates of Analysis (COA) with detailed chromatographic data are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to maintain stability and reactivity. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; Slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.