Description

L-4-Hydroxyphenyl-D4-Alanine-D1 is a stable isotope-labeled derivative of the amino acid L-tyrosine, specifically deuterated at four positions on the phenyl ring and one on the alanine moiety. This high-purity labeled compound is essential for advanced analytical research requiring precise internal standards and metabolic pathway tracing. It is primarily utilized by pharmaceutical R&D laboratories, contract research organizations (CROs), and academic institutions engaged in pharmacokinetic studies, proteomics, and biomarker discovery using mass spectrometry.

Application

• Internal Standard for LC-MS/MS: Serves as a critical quantitative internal standard in Liquid Chromatography-Tandem Mass Spectrometry for the accurate measurement of L-tyrosine and related metabolites in complex biological matrices.
• Metabolic Pathway Tracing: Used in deuterium-labeling studies to investigate tyrosine metabolism, protein synthesis rates, and neurotransmitter (dopamine, epinephrine) biosynthesis pathways.
• Proteomics & Biomarker Research: Enables precise relative and absolute quantification (AQUA) in proteomic workflows to identify and validate disease biomarkers.
• Drug Metabolism and Pharmacokinetics (DMPK): Facilitates the study of the absorption, distribution, metabolism, and excretion (ADME) of tyrosine-based drug candidates.
• Nutritional and Clinical Research: Applied in studies examining amino acid turnover, inborn errors of metabolism (e.g., tyrosinemia), and nutritional status assessment.
• Synthesis of Labeled Compounds: Acts as a key chiral building block for the synthesis of more complex deuterated pharmaceuticals or biochemical probes.

Basic Information

Product Name	L-4-Hydroxyphenyl-D4-Alanine-D1
CAS No.	1226919-57-8
Molecular Formula	C9H5D5NO3
Molecular Weight	186.22 g/mol (Calculated for deuterated form)
Synonyms	L-Tyrosine-d5; (2S)-2-Amino-3-(4-hydroxyphenyl-2,3,5,6-d4)propanoic-3,3-d2 acid; L-Tyrosine-3,3-d2, ring-2,3,5,6-d4; Deuterated L-Tyrosine; L-Tyr-d5; [2H5]-L-Tyrosine; (S)-2-Amino-3-(4-hydroxy-2,3,5,6-tetradeuterophenyl)-3,3-dideuteropropanoic acid
EINECS	Contact for details

Quality Control

Every batch of L-4-Hydroxyphenyl-D4-Alanine-D1 is manufactured and analyzed under strict quality management systems. We guarantee high chemical and isotopic purity, with comprehensive testing including HPLC for chemical purity, NMR and MS for structural confirmation and isotopic enrichment, and chiral analysis to verify enantiomeric purity. A detailed Certificate of Analysis (COA) is provided with each shipment, documenting all relevant specifications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, consider desiccation and storage under an inert atmosphere to maintain optimal stability and purity.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment	≥ 98 atom % D
Chemical Purity	≥ 95.0% (by NMR)
Optical Rotation	[α]20/D: -10.0° to -12.0° (c=1 in 1N HCl)
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.