Description

n-Tigloylglycine-2,2-D2 is a stable isotope-labeled analog of the endogenous metabolite n-tigloylglycine, specifically deuterated at the 2,2 position of the glycine moiety. This precise labeling makes it an indispensable internal standard for quantitative mass spectrometry, ensuring unparalleled accuracy in metabolic profiling and biomarker discovery. It is primarily utilized by research institutions, pharmaceutical companies, and clinical diagnostic laboratories engaged in advanced metabolomics, inborn errors of metabolism (IEM) research, and pharmacokinetic studies.

Application

• Quantitative Mass Spectrometry Internal Standard: Serves as a critical reference compound for the accurate quantification of endogenous n-tigloylglycine in complex biological matrices like plasma, urine, and tissue homogenates.
• Metabolomics & Biomarker Research: Enables precise tracking of fatty acid conjugation pathways, aiding in the discovery and validation of disease-specific metabolic signatures.
• Inborn Errors of Metabolism (IEM) Diagnostics: Used in clinical assays to diagnose and monitor disorders related to mitochondrial fatty acid oxidation and amino acid metabolism.
• Drug Metabolism & Pharmacokinetics (DMPK): Facilitates studies on drug-induced changes in metabolic pathways by providing a stable isotope-labeled tracer.
• Stable Isotope Dilution Analysis (SIDA): The gold-standard method for absolute quantification, where this compound is used to correct for analyte loss during sample preparation and instrument variability.
• Method Development & Validation: Essential for developing and validating robust LC-MS/MS or GC-MS methods in regulatory and research environments.

Basic Information

Product Name	n-Tigloylglycine-2,2-D2
CAS No.	1219806-44-6
Molecular Formula	C7H9D2NO3
Molecular Weight	159.18 g/mol (for deuterated form)
Synonyms	2,2-Dideutero-N-(2-methylbut-2-enoyl)glycine; 2-Methylcrotonoylglycine-2,2-d2; Tiglylglycine-2,2-d2; (E)-2-Methyl-2-butenoylglycine-2,2-d2; N-[(2E)-2-Methyl-2-butenoyl]glycine-2,2-d2; Deuterated n-Tigloylglycine; n-Tigloylglycine-d2; Stable Isotope Labeled n-Tigloylglycine
EINECS	Contact for details

Quality Control

Every batch of n-Tigloylglycine-2,2-D2 is manufactured and analyzed under strict quality systems to ensure identity, purity, and isotopic enrichment meet the exacting standards required for analytical applications. We provide comprehensive Certificates of Analysis (COA) detailing specifications for isotopic purity (e.g., D ≥ 98%), chemical purity (typically ≥95% by HPLC), and confirmation of structure by NMR and MS. Our quality commitment supports compliance with ISO 17025 and GLP principles for research and diagnostic use.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry, cool place. This compound is hygroscopic (moisture-sensitive); containers should be allowed to equilibrate to room temperature before opening to minimize moisture ingress.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (NMR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Isotopic Purity (D)	≥ 98 atom % D
Isotopic Enrichment (MS)	≥ 98% (M+2)
Water Content (KF)	≤ 2.0%
Residual Solvents (GC)	Complies with ICH limits

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.