Description

Hydrochlorothiazide-3,3-D2 is a deuterated isotopologue of the widely prescribed diuretic and antihypertensive agent hydrochlorothiazide. This labeled compound is of critical importance for use as an internal standard in quantitative bioanalytical methods, ensuring the highest accuracy and reliability in pharmacokinetic and metabolism studies. It is an essential tool for pharmaceutical R&D laboratories, contract research organizations (CROs), and analytical service providers engaged in drug development and regulatory compliance testing.

Application

• Internal Standard for LC-MS/MS: Primary application as a stable, non-interfering internal standard for the precise quantification of hydrochlorothiazide in complex biological matrices like plasma, serum, and urine.
• Pharmacokinetic & Metabolism Studies: Enables accurate tracking of drug absorption, distribution, metabolism, and excretion (ADME) profiles in preclinical and clinical research.
• Bioanalytical Method Development & Validation: Used to develop, optimize, and validate robust analytical methods compliant with FDA, EMA, and ICH guidelines.
• Drug-Drug Interaction Studies: Facilitates reliable analysis in studies investigating potential interactions between hydrochlorothiazide and other co-administered therapeutics.
• Reference Material for Certified Standards: Serves as a high-purity starting material for manufacturers of certified reference materials (CRMs).
• Isotope Dilution Mass Spectrometry (IDMS): Employed in high-precision analytical techniques that require isotope-labeled analogs for maximum accuracy.

Basic Information

Item	Detail
Product Name	Hydrochlorothiazide-3,3-D2
CAS No.	1219798-89-6
Molecular Formula	C₇H₆D₂ClN₃O₄S₂
Molecular Weight	299.21 g/mol (approx.)
Synonyms	6-Chloro-3,4-dihydro-2H-1,2,4-benzothiadiazine-7-sulfonamide 1,1-dioxide-3,3-d2; HCTZ-d2; Deuterated Hydrochlorothiazide; [3,3-D2]Hydrochlorothiazide; 3,3-Dideutero-hydrochlorothiazide; Esidrex-d2; Dichlotride-d2
EINECS	Contact for details

Quality Control

Every batch of Hydrochlorothiazide-3,3-D2 is manufactured and analyzed under strict quality systems. We guarantee high chemical and isotopic purity, confirmed by advanced spectroscopic and chromatographic techniques including NMR, HPLC, and MS. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing batch-specific results for identity, purity (HPLC assay), isotopic enrichment, and residual solvents. Our quality commitment supports compliance with the stringent requirements of GMP/GLP environments.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) in a dry environment. The product is light-sensitive (store away from light).

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥98.0%
Isotopic Enrichment	≥98 atom % D
Chemical Purity (NMR)	Conforms
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.