Description

5-Methyl-D3-Cytosine-6-D1 is a stable isotope-labeled derivative of the nucleobase cytosine, specifically deuterated at key positions. This compound is of critical importance for use as an internal standard in quantitative mass spectrometry, ensuring high accuracy and precision in analytical measurements. It is primarily needed by researchers and manufacturers in the fields of pharmaceutical development, metabolomics, and forensic analysis for tracing metabolic pathways and quantifying biomarkers.

Application

• Mass Spectrometry Internal Standard: Essential for the accurate quantification of cytosine, 5-methylcytosine, and related metabolites in complex biological matrices via LC-MS/MS and GC-MS.
• Epigenetics & DNA Methylation Research: Used in studies investigating DNA methylation patterns, a key epigenetic marker linked to gene expression and disease states like cancer.
• Pharmaceutical R&D: Employed in drug metabolism and pharmacokinetics (DMPK) studies to track the fate of nucleoside-based drug candidates.
• Clinical Diagnostics: Serves as a reference standard in diagnostic assays for detecting abnormal levels of modified nucleosides, which can be indicators of disease.
• Nutritional & Metabolic Studies: Facilitates precise measurement of one-carbon metabolism and folate cycle intermediates in nutritional science.
• Forensic Toxicology: Aids in the definitive identification and quantification of nucleoside analogs in forensic samples.

Basic Information

Product Name	5-Methyl-D3-Cytosine-6-D1
CAS No.	1219795-15-9
Molecular Formula	C5H4D4N3O
Molecular Weight	130.16 g/mol
Synonyms	5-Methylcytosine-d4; 5-Methylcytosine (methyl-D3, 6-D1); 4-Amino-5-methyl-D3-2(1H)-pyrimidinone-6-D1; 5-MC-d4; [Methyl-D3, 6-D1]-5-methylcytosine; 5-Hydroxymethylcytosine-d4 precursor; Isotopically labeled cytosine derivative; Stable isotope-labeled nucleobase
EINECS	Contact for details

Quality Control

Our 5-Methyl-D3-Cytosine-6-D1 is manufactured under strict quality systems to ensure the high isotopic purity and chemical integrity required for sensitive analytical applications. Each batch is characterized by advanced spectroscopic and chromatographic methods, including NMR and HPLC-MS, to confirm isotopic enrichment and chemical purity. A comprehensive Certificate of Analysis (COA) detailing isotopic purity (e.g., D ≥ 98%), chemical purity, and analytical data is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) in a dry environment. This material is hygroscopic (moisture-sensitive); containers must be kept tightly sealed to prevent degradation from atmospheric moisture.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (D)	≥ 98 atom % D
Isotopic Enrichment (MS)	≥ 95% (M+4)
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.