Description

n-Tris(Hydroxymethyl)Methyl-2-Aminoethanesulfonic Acid-D15 is a deuterated analog of the common biological buffer TES, where fifteen hydrogen atoms are replaced with deuterium. This high isotopic purity compound is critical for applications requiring minimal interference from proton signals, such as in advanced NMR spectroscopy and mass spectrometry. It is primarily utilized by research institutions, pharmaceutical developers, and analytical laboratories engaged in metabolic studies, protein structure analysis, and quantitative bioanalytical method development.

Application

• Internal Standard for Quantitative Mass Spectrometry (MS): Serves as a stable, non-interfering internal standard in LC-MS and GC-MS analyses for the accurate quantification of TES or related compounds in complex biological matrices.
• NMR Spectroscopy Solvent & Buffer: Used in deuterated NMR solvents to prepare buffers for studying protein dynamics, ligand binding, and metabolic pathways without background proton signal interference.
• Metabolomics and Stable Isotope Tracing: Employed in metabolic flux analysis and tracer studies to investigate biochemical pathways and enzyme kinetics in cellular systems.
• Pharmaceutical Research & Development: Facilitates drug metabolism and pharmacokinetics (DMPK) studies, providing a deuterated reference for analyzing drug compounds and their metabolites.
• Biochemical Assay Development: Acts as a deuterated buffer component in specialized enzymatic and binding assays where proton background must be minimized.
• Structural Biology: Aids in the structural elucidation of biomolecules via NMR, providing a stable isotopic environment for resonance assignment and distance constraint measurements.

Basic Information

Product Name	n-Tris(Hydroxymethyl)Methyl-2-Aminoethanesulfonic Acid-D15
CAS No.	1219794-63-4
Molecular Formula	C6D15NO6S
Molecular Weight	~259.33 g/mol
Synonyms	TES-d15; 2-[[1,1-Bis(hydroxymethyl)-2,2,2-trideuterioethoxy]methyl]amino]ethanesulfonic Acid-d12; Deuterated TES; (2-Hydroxy-1,1-bis(hydroxymethyl)ethyl)amino]methanesulfonic Acid-d15; N-[Tris(hydroxymethyl)methyl]-2-aminoethanesulfonic Acid-d15; Tris-D15 Hydroxymethyl Methyl Aminomethanesulfonic Acid; D15-TES; Deuterated Biological Buffer
EINECS	Contact for details

Quality Control

Every batch of n-Tris(Hydroxymethyl)Methyl-2-Aminoethanesulfonic Acid-D15 is manufactured and tested under strict quality management systems. We guarantee high isotopic purity (typically ≥98 atom % D) and chemical purity suitable for sensitive analytical applications. Certificates of Analysis (COA) are provided with each shipment, detailing specifications for isotopic enrichment, assay, identity, and purity by NMR and HPLC. Our quality commitment ensures consistency and reliability for your critical research and development needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent absorption of atmospheric moisture, which could affect isotopic purity and stability.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (NMR)	Conforms to structure
Isotopic Purity (Atom % D)	≥98%
Assay (HPLC)	≥98.0%
Chemical Purity (NMR)	≥98.0%
Water Content (KF)	≤1.0%
Residue on Ignition	≤0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.