Description

Biotin-[D2] (Vitamin H-[D2]) is a high-purity, deuterium-labeled isotopologue of the essential water-soluble vitamin, biotin. This stable isotope-labeled compound is critical for advanced analytical and metabolic research, providing a reliable internal standard for precise quantification. It is primarily utilized by pharmaceutical R&D laboratories, contract research organizations (CROs), and academic institutions engaged in mass spectrometry-based studies, pharmacokinetics, and metabolic pathway analysis.

Application

• Internal Standard for LC-MS/MS and GC-MS analysis in bioanalytical method development and validation.
• Quantitative analysis of biotin and its metabolites in complex biological matrices (e.g., plasma, serum, tissues).
• Tracer studies for investigating vitamin metabolism, absorption, and bioavailability in nutritional and pharmaceutical research.
• Use in drug discovery and development for pharmacokinetic (PK) and pharmacodynamic (PD) studies of biotin-conjugated therapeutics.
• High-accuracy calibration in clinical diagnostics and quality control laboratories for biotin assay kits.
• Metabolic flux analysis and stable isotope-resolved metabolomics (SIRM) studies.

Basic Information

Product Name	Biotin-[D2] (Vitamin H-[D2])
CAS No.	1217481-41-8
Molecular Formula	C10H14D2N2O3S
Molecular Weight	246.33 g/mol (approx.)
Synonyms	Vitamin H-[D2]; d2-Biotin; (+)-Biotin-d2; Hexahydro-2-oxo-1H-thieno[3,4-d]imidazole-4-pentanoic-3,4-d2 acid; cis-Hexahydro-2-oxo-1H-thieno[3,4]imidazole-4-valeric-3,4-d2 acid; Coenzyme R-d2; D-Biotin-d2; Biotin (ring-3,4-d2)
EINECS	Contact for details

Quality Control

Our Biotin-[D2] is manufactured under strict quality systems to ensure the highest isotopic and chemical purity for research applications. Each batch is characterized by advanced analytical techniques including NMR, HPLC, and mass spectrometry to confirm isotopic enrichment and chemical identity. A comprehensive Certificate of Analysis (COA) detailing purity, isotopic purity (D-enrichment), and analytical data is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive). For long-term storage, consider storage under an inert atmosphere or in a desiccator to maintain stability.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (D-enrichment)	≥ 98 atom % D
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.