Description

D-Phenyl-D5-Alanine-2,3,3-D3 is a stable isotope-labeled analog of the essential amino acid L-phenylalanine, specifically deuterated at multiple positions. This high-purity compound is critical for applications requiring precise molecular tracking and quantification, offering exceptional isotopic enrichment and chemical purity. It is primarily utilized by researchers and manufacturers in the pharmaceutical, biotechnology, and analytical chemistry sectors for advanced metabolic studies, proteomics, and as an internal standard in quantitative mass spectrometry.

Application

• Mass Spectrometry Internal Standard: Serves as a critical, non-interfering reference compound for the accurate quantification of phenylalanine and related metabolites in complex biological matrices using LC-MS/MS and GC-MS.
• Metabolic Pathway Tracing: Used in pharmacokinetic (PK) and pharmacodynamic (PD) studies to elucidate drug metabolism, amino acid turnover, and protein synthesis rates in vivo.
• Proteomics and Biomarker Research: Enables precise, multiplexed relative and absolute quantification (SILAC, AQUA) in bottom-up proteomics for disease biomarker discovery and validation.
• Pharmaceutical Development: Employed in the synthesis of deuterated drug candidates, where deuterium incorporation can improve metabolic stability and alter pharmacokinetic profiles.
• Nutritional and Clinical Research: Facilitates studies on phenylalanine metabolism in conditions like phenylketonuria (PKU) and other metabolic disorders.
• NMR Spectroscopy: Acts as a useful probe in nuclear magnetic resonance studies due to the distinct properties of deuterium nuclei.

Basic Information

Product Name	D-Phenyl-D5-Alanine-2,3,3-D3
CAS No.	1202064-02-5
Molecular Formula	C9D8NO2
Molecular Weight	~182.24 g/mol
Synonyms	(2R)-2-Amino-3-(2,3,4,5,6-pentadeuteriophenyl)propanoic-2,3,3-d3 acid; D-Phenylalanine-d8; (R)-Phenylalanine-d8; D-Phe-d8; Deuterated D-Phenylalanine; [D8]-D-Phenylalanine; (R)-2-Amino-3-(pentadeuteriophenyl)propionic-2,3,3-d3 acid
EINECS	Contact for details

Quality Control

Every batch of D-Phenyl-D5-Alanine-2,3,3-D3 is manufactured under controlled conditions and subjected to rigorous analytical testing to ensure it meets the stringent requirements for isotope-labeled standards. Our quality assurance protocol includes verification of isotopic purity (D-enrichment) and chemical purity. A comprehensive Certificate of Analysis (COA) detailing specifications such as assay by HPLC, isotopic enrichment by MS, chiral purity, and residual solvent content is provided with each shipment to guarantee traceability and compliance for your research and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) in a cool, dry place. The product is light-sensitive and should be handled to minimize prolonged exposure to light.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0% (Chemical Purity)
Isotopic Enrichment (MS)	≥ 98 atom % D
Chiral Purity (HPLC)	≥ 99.0% D-isomer
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.