Description

Glycocholic Acid-2,2,4,4-D4 is a high-purity, deuterium-labeled internal standard of the primary bile acid glycocholic acid, essential for precise quantitative analysis in mass spectrometry. This stable isotope-labeled compound is critical for ensuring accuracy and reliability in complex biological assays by correcting for analyte loss during sample preparation and instrument variability. It is primarily required by pharmaceutical R&D laboratories, clinical research organizations, and metabolomics facilities engaged in biomarker discovery, drug metabolism and pharmacokinetics (DMPK) studies, and in-depth investigations of liver function and bile acid disorders.

Application

• Internal Standard for LC-MS/MS: Used as a quantitative reference standard in liquid chromatography-tandem mass spectrometry for the accurate measurement of endogenous glycocholic acid and related bile acids in biological matrices like plasma, serum, and urine.
• Metabolomics and Biomarker Research: Enables precise profiling of bile acid metabolism in studies of liver diseases (cholestasis, NAFLD, cirrhosis), gastrointestinal disorders, and metabolic syndromes.
• Drug Development (DMPK): Critical in pharmaceutical research for studying the pharmacokinetics, bioavailability, and hepatobiliary excretion of new drug candidates, particularly those affecting bile acid pathways.
• Clinical Diagnostics: Supports the development and validation of high-sensitivity diagnostic assays for inborn errors of bile acid metabolism and other hepatobiliary conditions.
• Stable Isotope Dilution Analysis (SIDA): Serves as the cornerstone for this gold-standard quantitative technique, ensuring the highest level of accuracy in complex sample analysis.
• Quality Control in Reference Material Production: Employed in the calibration and verification of certified reference materials (CRMs) and assay kits for bile acid analysis.

Basic Information

Product Name	Glycocholic Acid-2,2,4,4-D4
CAS No.	1201918-15-1
Molecular Formula	C₂₆H₃₉D₄NO₆
Molecular Weight	~469.62 g/mol
Synonyms	N-[(3α,5β,7α,12α)-3,7,12-Trihydroxy-24-oxocholan-24-yl]glycine-2,2,4,4-d4; Deuterated Glycocholic Acid; Glycocholic Acid-d4; Cholylglycine-d4; 3α,7α,12α-Trihydroxy-5β-cholan-24-oylglycine-d4; N-Choloylglycine-d4; GA-d4; GCA-d4
EINECS	Contact for details

Quality Control

Our deuterated analytical standards are manufactured under strict quality systems to ensure the highest isotopic purity and chemical stability. Each batch undergoes comprehensive analysis, including HPLC for chemical purity and mass spectrometry for isotopic enrichment verification. Certificates of Analysis (COA) are provided, detailing lot-specific data on assay, isotopic purity, and solvent content. Production adheres to relevant guidelines for reference material preparation, supporting traceability and reliability in regulated environments.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. This compound is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent moisture absorption. For prolonged storage under an inert atmosphere, please contact us.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (MS)	Complies
Identification (NMR)	Complies
Purity (HPLC)	≥98.0%
Isotopic Purity (by MS)	≥98.0 atom % D
Chemical Purity (by NMR)	≥95.0%
Water Content (KF)	≤2.0%
Residual Solvents (GC)	Complies with ICH limits

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.