Description

Resorufin-[D6] is a deuterium-labeled analog of the fluorescent dye resorufin, specifically designed for use as an internal standard in quantitative mass spectrometry. This stable isotope-labeled compound is essential for ensuring accuracy and precision in analytical methods by correcting for variations during sample preparation and analysis. It is a critical reagent for researchers and quality control laboratories in the pharmaceutical, biotechnology, and environmental testing sectors who require reliable quantification of resorufin or its derivatives.

Application

• Internal Standard for LC-MS/MS quantification of resorufin in biological matrices (e.g., plasma, tissue homogenates).
• Metabolite tracking and pharmacokinetic studies of resorufin-based compounds or prodrugs.
• Calibration standard in high-performance liquid chromatography (HPLC) and fluorescence detection methods.
• Biomarker research and assay development involving enzyme activity (e.g., cytochrome P450, azoreductase) where resorufin is a product.
• Quality control and method validation in pharmaceutical analysis to meet regulatory standards (FDA, ICH).
• Environmental analysis for detecting and quantifying specific pollutants or enzymatic activities.

Basic Information

Product Name	Resorufin-[D6]
CAS No.	1196157-65-9
Molecular Formula	C12H1D6NO3
Molecular Weight	~219.20 g/mol
Synonyms	7-Hydroxy-3H-phenoxazin-3-one-d6; 3H-Phenoxazin-3-one, 7-hydroxy-d6; Deuterated Resorufin; Resorufin-d6; Resorufin (hexadeuterated); 7-Hydroxyphenoxazin-3-one-d6; Stable Isotope Labeled Resorufin
EINECS	Contact for details

Quality Control

Our Resorufin-[D6] is manufactured under strict quality control protocols to ensure high chemical purity and isotopic enrichment suitable for sensitive analytical applications. Each batch is characterized by advanced spectroscopic and chromatographic techniques, including NMR, HPLC, and mass spectrometry. A comprehensive Certificate of Analysis (COA) detailing purity, isotopic purity (D-enrichment), and analytical data is provided to support your research and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C or as indicated on the label/Certificate of Analysis. For long-term storage, keep the container in a cool, dry, and well-ventilated area.

Specification

Item	Specification
Appearance	Orange to red powder or crystalline solid
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 98.0%
Isotopic Purity (D-enrichment)	≥ 98 atom % D
Chemical Purity (NMR)	Conforms to structure
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.