Description

2'-Fluoro-2'-Deoxy-Ara-U-3'-Phosphoramidite is a critical phosphoramidite monomer used in the automated solid-phase synthesis of oligonucleotides. This compound is essential for introducing a 2'-fluoro modification on an arabinose sugar backbone, which significantly enhances the nuclease resistance and binding affinity of therapeutic oligonucleotides. It is a key building block for research and development in antisense therapy, siRNA, and diagnostic probe manufacturing, primarily serving pharmaceutical R&D, biotechnology firms, and CROs focused on next-generation nucleic acid therapeutics.

Application

• Synthesis of antisense oligonucleotides (ASOs) with improved metabolic stability.
• Manufacture of small interfering RNA (siRNA) constructs for gene silencing applications.
• Production of diagnostic probes and primers requiring enhanced specificity and duplex stability.
• Research into aptamer development for therapeutic and sensing platforms.
• Solid-phase synthesis of modified DNA/RNA strands for structure-activity relationship (SAR) studies.
• Preparation of oligonucleotides for use in crystallography and NMR studies due to the stabilizing 2'-fluoro modification.

Basic Information

Product Name	2'-Fluoro-2'-Deoxy-Ara-U-3'-Phosphoramidite
CAS No.	1190089-70-3
Molecular Formula	C33H41FN3O7P
Molecular Weight	641.67 g/mol
Synonyms	2'-F-Ara-U Phosphoramidite; 1-[3,5-Bis-O-(4-chlorophenyl)phenyl-2-fluoro-2-deoxy-β-D-arabinofuranosyl]uracil 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; 2'-Fluoro-2'-deoxyarabinouridine 3'-phosphoramidite; 5'-O-DMT-2'-F-araU-3'-phosphoramidite; 2'-F-araU Phosphoramidite; 2'-Fluoro-arabino-Uridine Phosphoramidite
EINECS	Contact for details

Quality Control

Every batch of 2'-Fluoro-2'-Deoxy-Ara-U-3'-Phosphoramidite is manufactured and tested under strict quality systems. We provide comprehensive Certificates of Analysis (COA) detailing purity, identity, and moisture content, ensuring compliance with the exacting standards required for oligonucleotide synthesis. Our quality commitment supports applications in GMP-grade therapeutic development and cGMP manufacturing environments.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dry, moisture-free environment. Due to its hygroscopic and moisture-sensitive nature, the product must be handled in a controlled, dry atmosphere and allowed to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with reference structure
Purity (HPLC)	≥ 97.0%
Moisture Content (KF)	≤ 1.0%
Solubility	Soluble in anhydrous acetonitrile, dichloromethane

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.