Description

7-Ethoxycoumarin-D5 is a deuterium-labeled analog of 7-ethoxycoumarin, a well-established substrate for cytochrome P450 enzyme activity assays. This stable isotope-labeled compound is essential for use as an internal standard in quantitative bioanalytical methods, ensuring high accuracy and precision in mass spectrometry-based analyses. It is primarily utilized by pharmaceutical R&D laboratories, contract research organizations (CROs), and academic institutions engaged in drug metabolism and pharmacokinetics (DMPK) studies.

Application

• Internal Standard for LC-MS/MS: Critical for the accurate quantification of 7-ethoxycoumarin and its metabolites in in vitro and in vivo biological samples.
• Drug Metabolism Studies: Used in enzyme kinetic assays to measure the activity of specific cytochrome P450 isoforms, particularly CYP1A2 and CYP2E1.
• High-Throughput Screening (HTS): Employed in pharmaceutical discovery for assessing the inhibitory or inductive potential of new chemical entities on metabolic enzymes.
• Toxicological Research: Facilitates studies on xenobiotic metabolism and bioactivation pathways in liver microsomes and hepatocytes.
• Biomarker Analysis: Supports method development for clinical research investigating individual metabolic phenotypes.
• Quality Control in Bioanalysis: Ensures reliability and compliance with regulatory standards (e.g., FDA, EMA) for bioanalytical method validation.

Basic Information

Product Name	7-Ethoxycoumarin-D5
CAS No.	1189956-39-5
Molecular Formula	C11D5H5O3
Molecular Weight	~195.20 g/mol
Synonyms	7-Ethoxy-2H-1-benzopyran-2-one-d5; 7-Ethoxy-2-oxo-2H-chromene-d5; 7-Ethoxycoumarin (ethyl-d5); 7-EC-d5; Deuterated 7-ethoxycoumarin; 7-Ethoxy-2H5coumarin; CYP1A2 Substrate-d5
EINECS	Contact for details

Quality Control

Our 7-Ethoxycoumarin-D5 is manufactured under controlled conditions to ensure exceptional isotopic and chemical purity, critical for its role as an analytical standard. Each batch is supported by a comprehensive Certificate of Analysis (COA) detailing results for isotopic purity (D-enrichment), chemical purity (by HPLC), and identity confirmation (by NMR and MS). We adhere to rigorous quality testing protocols suitable for research and regulatory submission support.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a cool, dry place. This product is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥98.0% (chemical purity)
Isotopic Purity (D-enrichment)	≥98 atom % D
Water Content (KF)	≤0.5%
Residue on Ignition	≤0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.