Description

Trimethoprim-D3 is a deuterium-labeled analog of the antibiotic Trimethoprim, specifically designed for use as an internal standard in quantitative mass spectrometry. This high-purity compound is essential for ensuring accurate and reliable analytical results in pharmacokinetic and metabolic studies. It is primarily utilized by pharmaceutical R&D laboratories, contract research organizations (CROs), and analytical service providers engaged in drug development and bioanalytical method validation.

Application

• Quantitative Bioanalysis: Serves as a critical internal standard in LC-MS/MS methods for the precise quantification of Trimethoprim and its metabolites in biological matrices such as plasma, serum, and urine.
• Drug Metabolism and Pharmacokinetics (DMPK): Used in tracer studies to investigate the absorption, distribution, metabolism, and excretion (ADME) profile of Trimethoprim.
• Clinical Research: Enables accurate therapeutic drug monitoring (TDM) in clinical studies to correlate drug levels with efficacy and safety outcomes.
• Method Development and Validation: A key component in developing and validating robust, regulatory-compliant analytical methods for GLP and GCP studies.
• Pharmaceutical Quality Control: Can be employed in impurity profiling and stability-indicating assays for Trimethoprim drug substances and products.

Basic Information

Item	Detail
Product Name	Trimethoprim-D3
CAS No.	1189923-38-3
Molecular Formula	C14H15D3N4O3
Molecular Weight	293.33 g/mol
Synonyms	2,4-Diamino-5-(3,4,5-trimethoxybenzyl)pyrimidine-D3; 5-[(3,4,5-Trimethoxyphenyl)methyl]-2,4-pyrimidinediamine-D3; Trimethoprim (methyl-D3); Deuterated Trimethoprim; Trimethoprim-d3; [2H3]-Trimethoprim
EINECS	Contact for details

Quality Control

Our Trimethoprim-D3 is manufactured under strict quality control protocols to ensure the highest standards of isotopic purity and chemical integrity, which are paramount for its use as an analytical standard. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing results for isotopic enrichment (D-content), chemical purity (by HPLC), and identity confirmation (by NMR and MS). We adhere to relevant guidelines for the production of labeled compounds for research use.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation. For long-term storage, consider desiccation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (D-content)	≥ 98 atom % D
Chemical Purity	≥ 95% (by NMR)
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.