Description

5A-Cholest-7-En-3-Ol-1,2,5A,6A-D4 is a deuterated sterol derivative, specifically a labeled analog of a cholesterol-related intermediate. This compound is of significant value for its utility as a stable isotope-labeled internal standard in advanced analytical chemistry. It is primarily required by research institutions and pharmaceutical companies engaged in metabolic studies, lipidomics research, and the development of analytical methods using techniques like LC-MS/MS and GC-MS to ensure precise quantification and traceability.

Application

• Internal Standard for Mass Spectrometry: Serves as a critical deuterium-labeled reference compound in quantitative LC-MS and GC-MS analysis of sterols and related metabolites.
• Biochemical Research: Used in tracer studies to investigate cholesterol biosynthesis pathways, metabolic flux, and enzyme kinetics.
• Pharmaceutical Development: Employed in drug metabolism and pharmacokinetics (DMPK) studies for candidate drugs targeting lipid pathways.
• Diagnostic Assay Development: Utilized in the calibration and validation of clinical diagnostic assays measuring sterol levels.
• Lipidomics: A key tool in high-throughput lipid profiling to accurately identify and quantify specific sterol species in complex biological samples.
• Quality Control Reference: Acts as a certified reference material for ensuring the accuracy and precision of analytical results in quality control laboratories.

Basic Information

Product Name	5A-Cholest-7-En-3-Ol-1,2,5A,6A-D4
CAS No.	1182005-25-9
Molecular Formula	C27H42D4O
Molecular Weight	390.66 g/mol (approx.)
Synonyms	Cholest-7-en-3-ol-1,2,5α,6α-d4 (5α); 1,2,5α,6α-Tetradeutero-5α-cholest-7-en-3β-ol; 5α-Cholest-7-ene-3β-ol-1,2,5α,6α-d4; Deuterated Lathosterol-d4 (isomer); [1,2,5α,6α-2H4]-5α-Cholest-7-en-3β-ol; 3β-Hydroxy-5α-cholest-7-ene-1,2,5α,6α-d4
EINECS	Contact for details

Quality Control

Our 5A-Cholest-7-En-3-Ol-1,2,5A,6A-D4 is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including NMR and mass spectrometry, to confirm isotopic enrichment, chemical purity, and structural identity. Certificates of Analysis (COA) detailing batch-specific results for purity, isotopic purity (D-enrichment), and solvent residues are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C or as indicated on the label or COA. This product is hygroscopic (moisture-sensitive) and light-sensitive; appropriate handling to minimize exposure to air and light is recommended for long-term stability.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Isotopic Purity (MS)	≥ 98 atom % D
Chemical Purity (NMR)	Conforms
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.