Description

Pyridoxamine-[D3] Dihydrochloride (Vitamin B6-[D3]) is a high-purity, deuterium-labeled isotopologue of pyridoxamine dihydrochloride, a vital form of Vitamin B6. This stable isotope-labeled compound is essential for advanced analytical research, providing an internal standard for precise quantification and metabolic pathway tracing in complex biological matrices. It is primarily utilized by research scientists and quality control laboratories in the pharmaceutical, nutraceutical, and clinical diagnostics industries where accurate measurement of Vitamin B6 and its metabolites is critical.

Application

• Internal Standard for LC-MS/MS: Serves as a critical reference compound in liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the accurate quantification of pyridoxamine and related Vitamin B6 vitamers in biological samples like plasma, serum, and tissues.
• Metabolic Research & Tracer Studies: Used in pharmacokinetic, pharmacodynamic, and metabolic flux analysis studies to investigate the absorption, distribution, metabolism, and excretion (ADME) of Vitamin B6.
• Pharmaceutical Quality Control: Employed in the assay and impurity profiling of pharmaceutical formulations containing Vitamin B6 to ensure potency, purity, and compliance with regulatory standards (e.g., USP, EP).
• Clinical Diagnostics Development: Facilitates the development and validation of robust clinical assays for monitoring Vitamin B6 status, which is linked to cardiovascular, neurological, and metabolic health.
• Nutraceutical & Food Analysis: Enables precise measurement of Vitamin B6 content and its degradation products in fortified foods, dietary supplements, and infant formula.
• Biochemical Research: Acts as a tool in enzymology studies to understand the role of pyridoxamine in enzymatic reactions as a cofactor.

Basic Information

Product Name	Pyridoxamine-[D3] Dihydrochloride (Vitamin B6-[D3])
CAS No.	1173023-45-4
Molecular Formula	C8H9D3Cl2N2O2 • (HCl)2
Molecular Weight	291.60 g/mol (for monoisotopic mass)
Synonyms	4-(Aminomethyl)-5-(hydroxymethyl)-2-methylpyridin-3-ol-[D3] Dihydrochloride; Deuterated Pyridoxamine Dihydrochloride; Vitamin B6 D3; Pyridoxamine-d3 2HCl; 2-Methyl-3-hydroxy-4-aminomethyl-5-hydroxymethylpyridine-[D3] Dihydrochloride; 3-Hydroxy-4-(aminomethyl)-5-(hydroxymethyl)-2-methylpyridine-[D3] Dihydrochloride
EINECS	Contact for details

Quality Control

Our Pyridoxamine-[D3] Dihydrochloride is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including NMR (¹H, ²H) for isotopic enrichment verification and HPLC for chemical and isotopic purity assessment. Certificates of Analysis (COA) detailing batch-specific results for identity, purity (typically ≥98% by HPLC), isotopic purity (≥98 atom % D), and residual solvents are provided. We support compliance with relevant guidelines for reference standards.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. This product is hygroscopic (moisture-sensitive). For long-term storage, consider desiccation. Keep container tightly sealed in a dry and well-ventilated place.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity	≥ 98 atom % D
Water Content (KF)	≤ 2.0%
Residual Solvents (GC)	Complies with ICH limits

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.