Description

5-Methyl-2'-Deoxy Cytidine-D3 is a stable isotope-labeled nucleoside analog, specifically deuterated at the methyl group, which is critical for use as an internal standard in quantitative mass spectrometry. This high-purity compound enables precise and accurate measurement in complex biological matrices, eliminating variability and ensuring data integrity. It is primarily required by pharmaceutical researchers, contract research organizations (CROs), and diagnostic developers working on nucleoside metabolism, epigenetic studies, and the development of antiviral or anticancer therapeutics.

Application

• Quantitative Bioanalysis (LC-MS/MS): Serves as a critical internal standard for the accurate quantification of endogenous 5-methyl-2'-deoxycytidine and related analogs in pharmacokinetic and metabolomic studies.
• Epigenetics and DNA Methylation Research: Used as a stable isotope tracer to investigate dynamic changes in DNA methylation patterns and the activity of DNA methyltransferases.
• Diagnostic Assay Development: Essential for calibrating and validating high-sensitivity clinical diagnostic tests that measure modified nucleosides as potential disease biomarkers.
• Pharmaceutical Quality Control: Employed in the purity and potency assessment of nucleoside-based active pharmaceutical ingredients (APIs) and finished drug products.
• Metabolic Pathway Tracing: Facilitates the study of nucleoside salvage pathways and the cellular uptake and incorporation of modified nucleosides.

Basic Information

Product Name	5-Methyl-2'-Deoxy Cytidine-D3
CAS No.	1160707-78-7
Molecular Formula	C₁₀H₁₂D₃N₃O₄
Molecular Weight	242.25 g/mol
Synonyms	5-Methyl-2'-deoxycytidine-d3; 5-Methyldeoxycytidine-d3; 5-Methyl-dC-d3; [Methyl-d3]-5-methyl-2'-deoxycytidine; 4-Amino-1-((2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-(trideuteriomethyl)pyrimidin-2(1H)-one; 5-mC-d3; Deuterated 5-Methyldeoxycytidine
EINECS	Contact for details

Quality Control

Our 5-Methyl-2'-Deoxy Cytidine-D3 is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including NMR, HPLC, and mass spectrometry, to confirm chemical identity, isotopic purity (D-enrichment), and chemical purity. We provide a detailed Certificate of Analysis (COA) with each shipment, which includes batch-specific data on purity, residual solvents, and heavy metals, ensuring compliance with research and development standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (D-enrichment)	≥ 98 atom % D
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.