Description

L-Leucine-D7-n-T-Boc H2O (iso-Propyl-D7) is a stable isotope-labeled derivative of the essential amino acid L-leucine, specifically deuterated on the tert-butyloxycarbonyl (Boc) protecting group's isopropyl moiety. This compound is critical for providing an internal standard with minimal isotopic interference in advanced mass spectrometry-based quantitative analyses. It is primarily required by research and development laboratories in the pharmaceutical, biotechnology, and metabolic research sectors for precise pharmacokinetic, proteomic, and metabolic flux studies.

Application

• Internal Standard for LC-MS/MS quantification of peptides, proteins, and metabolites in complex biological matrices.
• Tracer compound in Stable Isotope Labeling by Amino acids in Cell culture (SILAC) for quantitative proteomics.
• Use in pharmacokinetic (PK) and pharmacodynamic (PD) studies to track drug metabolism and amino acid incorporation.
• Critical reagent for developing diagnostic assays and biomarker validation in clinical research.
• Substrate in enzymatic and metabolic pathway analysis to study protein synthesis and turnover rates.
• Building block for the synthesis of deuterated peptides and other labeled bioactive molecules for research.

Basic Information

Product Name	L-Leucine-D7-n-T-Boc H2O (iso-Propyl-D7)
CAS No.	1159283-32-5
Molecular Formula	C₁₁H₁₂D₇NO₄
Molecular Weight	~250.34 g/mol
Synonyms	(2S)-2-[[(2-Methylpropan-2-yl)oxy]carbonylamino]-4-methylpentanoic acid (iso-Propyl-D7); N-(tert-Butoxycarbonyl)-L-leucine (iso-Propyl-D7); Boc-L-Leucine-D7 (iso-Propyl-D7); (S)-2-((tert-Butoxycarbonyl)amino)-4-methylpentanoic acid (iso-Propyl-D7); N-Boc-L-leucine-D7 (iso-Propyl-D7); Deuterated Boc-Leucine; L-Leucine, N-[(1,1-dimethylethoxy)carbonyl]-, (iso-Propyl-D7)
EINECS	Contact for details

Quality Control

Our L-Leucine-D7-n-T-Boc H2O (iso-Propyl-D7) is manufactured under strict quality systems to ensure high chemical and isotopic purity, essential for sensitive analytical applications. Each batch is characterized by advanced spectroscopic and chromatographic techniques, including NMR, HPLC, and MS, to confirm structure, deuterium incorporation, and purity. Certificates of Analysis (COA) providing detailed specifications for isotopic enrichment, chemical purity, and chiral purity are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a cool, dry place. This material is hygroscopic (moisture-sensitive). For long-term storage, consider desiccation or storage under an inert atmosphere to maintain stability and purity.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment (MS)	≥ 98 atom % D
Chiral Purity (HPLC)	≥ 99.0% (L-isomer)
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.