Description

[5,5-2H2]-L-Citrulline is a stable isotope-labeled analog of the amino acid L-Citrulline, specifically deuterated at the 5,5 positions. This high-purity labeled compound is essential for advanced research requiring precise metabolic and pharmacokinetic tracing. It is primarily utilized by pharmaceutical R&D, academic research institutions, and analytical laboratories engaged in mass spectrometry-based studies.

Application

• Internal Standard for Quantitative Analysis: Serves as a critical internal standard in Liquid Chromatography-Mass Spectrometry (LC-MS) and Gas Chromatography-Mass Spectrometry (GC-MS) for the accurate quantification of endogenous L-Citrulline in complex biological matrices.
• Metabolic Pathway Tracing: Used in stable isotope tracer studies to investigate the urea cycle, nitric oxide synthase pathway, and arginine metabolism in cell cultures, animal models, and clinical research.
• Drug Metabolism and Pharmacokinetics (DMPK): Employed in the development and validation of bioanalytical methods for novel therapeutics targeting pathways involving citrulline and arginine.
• Proteomics and Biomarker Discovery: Facilitates relative and absolute quantification in proteomic studies where protein citrullination (deimination) is a focus, such as in autoimmune disease research.
• Nutritional and Sports Science Research: Enables precise tracking of citrulline absorption, distribution, and metabolism in studies related to dietary supplements and athletic performance.
• Synthesis of Labeled Compounds: Acts as a labeled building block for the synthesis of more complex deuterated molecules for research and development purposes.

Basic Information

Product Name	[5,5-2H2]-L-Citrulline
CAS No.	1021439-17-7
Molecular Formula	C6H11D2N3O3
Molecular Weight	177.18 g/mol
Synonyms	(2S)-2-Amino-5-([(4S)-4-amino-4-carboxybutyl]carbamoyl)amino-5,5-dideuteriopentanoic acid; L-Citrulline-5,5-d2; (S)-2-Amino-5-ureidopentanoic-5,5-d2 acid; Deuterated L-Citrulline; δ-Ureido-α-aminovaleric-5,5-d2 acid; N5-(Carbamoyl)-L-ornithine-5,5-d2; Stable Isotope-Labeled L-Citrulline
EINECS	Contact for details

Quality Control

Every batch of [5,5-2H2]-L-Citrulline is manufactured and analyzed under strict quality systems to ensure the highest isotopic and chemical purity for research applications. Our products undergo rigorous quality testing to ensure compliance with industry standards. Certificates of Analysis (COA) detailing isotopic enrichment (D), chemical purity, and analytical methods (NMR, MS, HPLC) are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is hygroscopic (moisture-sensitive) and must be kept under inert conditions (e.g., nitrogen atmosphere) after opening to maintain stability and prevent degradation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment (MS)	≥ 98 atom % D
Chemical Purity (Titration)	≥ 97.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.