Description

3-Hydroxy-5-Methyl-n-Phenyl-2-1H-Pyridone-D5 is a deuterated isotopologue of a key pyridone derivative, where five hydrogen atoms are replaced with deuterium, making it an invaluable internal standard for quantitative mass spectrometry. This compound is critical for ensuring analytical accuracy and reliability in pharmacokinetic and metabolic studies. It is primarily utilized by pharmaceutical researchers, analytical laboratories, and contract research organizations (CROs) engaged in drug development and bioanalytical method validation.

Application

• Internal Standard for LC-MS/MS: Serves as a stable, non-interfering reference compound for the precise quantification of its non-deuterated counterpart in complex biological matrices like plasma, serum, and urine.
• Drug Metabolism and Pharmacokinetics (DMPK): Essential for tracing the absorption, distribution, metabolism, and excretion (ADME) profiles of pharmaceutical candidates in preclinical and clinical studies.
• Bioanalytical Method Development: Used to validate and optimize liquid chromatography-tandem mass spectrometry (LC-MS/MS) assays, improving method robustness, accuracy, and precision.
• Metabolite Identification and Profiling: Aids in distinguishing drug-derived metabolites from endogenous compounds in mass spectrometric analysis.
• Quality Control in Pharmaceutical Manufacturing: Supports impurity profiling and ensures batch-to-batch consistency of active pharmaceutical ingredients (APIs).
• Forensic and Clinical Toxicology: Enables accurate measurement of specific compounds or their metabolites in forensic analysis and therapeutic drug monitoring.

Basic Information

Product Name	3-Hydroxy-5-Methyl-n-Phenyl-2-1H-Pyridone-D5
CAS No.	1020719-53-2
Molecular Formula	C13H6D5NO2
Molecular Weight	~221.26 g/mol
Synonyms	3-Hydroxy-5-methyl-2-phenyl-4(1H)-pyridinone-d5; 3-Hydroxy-5-methyl-2-phenyl-4-pyridone-d5; 5-Methyl-3-hydroxy-2-phenyl-4(1H)-pyridinone-d5; 2-Phenyl-3-hydroxy-5-methyl-4(1H)-pyridinone-d5; Deuterated Pyridone Standard; D5-3-Hydroxy-5-methyl-2-phenyl-4-pyridone; (D5)-3-Hydroxy-5-methyl-2-phenyl-1H-pyridin-4-one
EINECS	Contact for details

Quality Control

Our deuterated standards are produced under strict quality management to ensure the highest isotopic purity and chemical integrity, which is paramount for accurate analytical results. Each batch is characterized using advanced techniques including NMR (¹H, ²H) and High-Resolution Mass Spectrometry (HRMS) to confirm deuteration level and identity. A comprehensive Certificate of Analysis (COA) is provided, detailing specifications for isotopic purity (e.g., D5 > 98 atom % D), chemical purity (by HPLC), and residual solvent content.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic (moisture-sensitive) nature, the container should be kept in a desiccated environment. For long-term stability, consider storage under an inert atmosphere such as argon or nitrogen.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (D5)	≥ 98 atom % D
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.