Description

N2-Isobutyryl-3'-(Trityl)Amino-2', 3'-Dideoxyguanosine-5'-Cyanoethyl Phosphoramidite is a high-purity, protected nucleoside phosphoramidite monomer essential for automated oligonucleotide synthesis. This compound is critical for the precise and efficient incorporation of modified guanosine bases into synthetic DNA and RNA strands, enabling the creation of advanced nucleic acid-based tools. It is primarily utilized by researchers and manufacturers in the fields of therapeutic oligonucleotide development, diagnostic probe synthesis, and basic biochemical research where sequence-specific functionality is paramount.

Application

• Solid-Phase Oligonucleotide Synthesis: As a key building block (monomer) in automated DNA/RNA synthesizers for the production of custom sequences.
• Antisense Oligonucleotide (ASO) Manufacturing: For the synthesis of therapeutic oligonucleotides targeting specific mRNA sequences.
• siRNA and miRNA Synthesis: Enabling the production of short interfering RNA and microRNA for gene silencing and functional genomics studies.
• Diagnostic Probe Development: Used to create labeled or modified probes for PCR, FISH, and microarray applications.
• Aptamer Production: For synthesizing libraries of nucleic acid aptamers used in biosensing and targeted therapeutics.
• Research-Grade Oligo Synthesis: Supplying academic and industrial R&D labs with the necessary monomers for exploratory nucleic acid chemistry.

Basic Information

Item	Details
Product Name	N2-Isobutyryl-3'-(Trityl)Amino-2', 3'-Dideoxyguanosine-5'-Cyanoethyl Phosphoramidite
CAS No.	195375-66-7
Molecular Formula	C46H51N6O6P
Molecular Weight	815.92 g/mol
Synonyms	2'-Deoxy-3'-amino-2',3'-dideoxyguanosine N2-isobutyryl-5'-O-[2-cyanoethyl-(N,N-diisopropyl)]phosphoramidite, 3'-Tritylamino-2',3'-dideoxyguanosine N2-isobutyryl-5'-O-(2-cyanoethyl-N,N-diisopropyl)phosphoramidite, 5'-O-(2-Cyanoethyl-N,N-diisopropylphosphoramidite)-2'-deoxy-3'-tritylamino-2',3'-dideoxy-N2-isobutyrylguanosine, dG(ib) Phosphoramidite (3'-amino modifier), Guanosine, 2',3'-dideoxy-2-[[(1,1-dimethylethoxy)carbonyl]amino]-, 5'-[2-[[bis(1-methylethyl)amino](2-cyanoethoxy)phosphino]oxy]-, 2-(2-methyl-1-oxopropanoate), 3'-(triphenylmethyl) ether
EINECS	Contact for details

Quality Control

Our N2-Isobutyryl-3'-(Trityl)Amino-2', 3'-Dideoxyguanosine-5'-Cyanoethyl Phosphoramidite is manufactured under strict quality systems to ensure consistency and reliability for critical synthesis applications. Each batch is subjected to rigorous analytical testing, including HPLC purity analysis, 31P NMR, and 1H NMR spectroscopy, to confirm identity, purity, and suitability for oligonucleotide synthesis. Certificates of Analysis (COA) with detailed chromatographic and spectroscopic data are provided to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dry, moisture-free environment. This product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to air, moisture, or ambient light will reduce its stability and coupling efficiency. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 97.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in anhydrous acetonitrile, dichloromethane

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.