Description

N4-Benzoyl-3'-(Trityl)Amino-2', 3'-Dideoxycytidine-5'-Cyanoethyl Phosphoramidite is a high-purity, protected phosphoramidite monomer essential for the automated solid-phase synthesis of oligonucleotides. This compound is critical for ensuring high coupling efficiency and sequence fidelity in the production of modified DNA and RNA strands. It is primarily utilized by research institutions, diagnostic developers, and pharmaceutical companies engaged in advanced genetic research, therapeutic oligonucleotide development, and molecular diagnostics.

Application

• Oligonucleotide Synthesis: As a key building block (monomer) in automated solid-phase synthesizers for the production of custom DNA and RNA sequences.
• Antisense & siRNA Therapeutics: For the synthesis of chemically modified oligonucleotides used in gene silencing and therapeutic applications.
• PCR Primers & Probes: Manufacturing of high-purity, sequence-specific primers and fluorescently labeled probes for diagnostic assays and genetic testing.
• Research & Development: Enabling academic and industrial R&D in genomics, molecular biology, and drug discovery projects requiring custom oligonucleotides.
• Diagnostic Kits: Production of critical components for in vitro diagnostic (IVD) kits, including those for infectious disease detection and genetic analysis.
• Aptamer Development: Synthesis of modified nucleic acid libraries for the selection of aptamers with therapeutic or diagnostic potential.

Basic Information

Product Name	N4-Benzoyl-3'-(Trityl)Amino-2', 3'-Dideoxycytidine-5'-Cyanoethyl Phosphoramidite
CAS No.	195375-65-6
Molecular Formula	C46H44N5O5P
Molecular Weight	785.85 g/mol
Synonyms	5'-O-(2-Cyanoethyl)-N,N-diisopropylphosphoramidite of N4-Benzoyl-3'-O-Trityl-2',3'-Dideoxycytidine; 2'-Deoxycytidine-5'-phosphoramidite, N-benzoyl-3'-O-trityl- derivative; dC(Bz) Phosphoramidite, 3'-O-Trityl; N4-Benzoyl-5'-O-[bis(1-methylethyl)amino](2-cyanoethoxy)phosphino]-3'-O-(triphenylmethyl)-2',3'-dideoxycytidine; Amidite dC(Bz) Trt; DNA Synthesis Phosphoramidite Monomer; 195375-65-6
EINECS	Contact for details

Quality Control

Every batch of N4-Benzoyl-3'-(Trityl)Amino-2', 3'-Dideoxycytidine-5'-Cyanoethyl Phosphoramidite is manufactured and tested under strict quality management systems to ensure it meets the exacting standards required for oligonucleotide synthesis. Our products undergo rigorous analytical testing, including HPLC, 31P NMR, and 1H NMR, to guarantee high purity, correct structure, and optimal performance. Certificates of Analysis (COA) with detailed chromatographic and spectroscopic data are provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dry, moisture-free environment. This product is hygroscopic (moisture-sensitive) and easily oxidized; prolonged exposure to air, light, or ambient humidity will compromise purity and reactivity. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Retention time corresponds to reference standard
Identification (31P NMR)	Spectrum corresponds to reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.