Description

3,4-Cyclohexenoesculetin β-D-Galactopyranoside is a specialized synthetic chromogenic substrate designed for high-sensitivity enzymatic detection. This compound matters for its role as a critical reagent in biochemical research and diagnostic assay development, offering precise and reliable results. It is primarily needed by manufacturers and research institutions in the life sciences, pharmaceutical, and in-vitro diagnostic (IVD) industries for applications requiring the detection of β-galactosidase activity.

Application

• Biochemical Research: Used as a sensitive substrate for the detection and quantification of β-galactosidase enzyme activity in cell lysates and purified enzyme preparations.
• Diagnostic Assay Development: A key component in the formulation of chromogenic kits for clinical diagnostics, including point-of-care testing and automated analyzer systems.
• Microbiology & Cell Biology: Employed in reporter gene assays (e.g., lacZ gene expression) to study gene regulation, promoter activity, and cellular signaling pathways.
• High-Throughput Screening (HTS): Utilized in pharmaceutical and biotechnology research for screening compound libraries against β-galactosidase-linked targets.
• Food Safety & Quality Control: Applied in enzymatic methods for the detection of specific microbial contaminants in food and environmental samples.
• IVD Manufacturing: Serves as a raw material in the production of standardized diagnostic reagents and calibrators.

Basic Information

Product Name	3,4-Cyclohexenoesculetin β-D-Galactopyranoside
CAS No.	182805-65-8
Molecular Formula	C19H22O10
Molecular Weight	410.37 g/mol
Synonyms	3,4-Cyclohexenoesculetin β-D-galactopyranoside; 6,7-Dihydroxy-4-methyl-2-oxo-2H-1-benzopyran-3-yl β-D-galactopyranoside; CHG; Cyclohexenoesculetin galactopyranoside; β-Galactosidase substrate CHG; Chromogenic β-galactosidase substrate; 3,4-CHG-β-Gal; 4-Methylumbelliferyl-β-D-galactopyranoside analog (structural).
EINECS	Contact for details

Quality Control

Our 3,4-Cyclohexenoesculetin β-D-Galactopyranoside is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and diagnostic applications. Certificates of Analysis (COA) with detailed specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) in a dry environment. This product is light-sensitive (store away from light).

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/IR)	Conforms to reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Residue on Ignition	≤ 0.5%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Functional Activity	Suitable for β-galactosidase detection

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.