Description

5''-(Methyl-D3-Thio)Adenosine is a stable isotope-labeled analog of the key metabolic intermediate S-adenosylmethionine (SAMe). This compound matters for researchers requiring precise tracking and quantification in biochemical studies, particularly those involving methylation pathways and enzyme kinetics. It is essential for scientists in pharmaceutical R&D, academic institutions, and contract research organizations (CROs) focused on metabolomics, drug metabolism studies, and the development of diagnostic assays.

Application

• Internal Standard for LC-MS/MS: Serves as a critical quantitative reference in liquid chromatography-mass spectrometry for analyzing endogenous SAMe and related metabolites.
• Metabolic Pathway Tracing: Used in stable isotope labeling experiments (SILE) to elucidate methylation and transsulfuration pathways in cell culture and in vivo models.
• Enzyme Mechanism Studies: Employed as a substrate or probe to investigate the kinetics and mechanisms of methyltransferases and other SAMe-dependent enzymes.
• Pharmaceutical Development: Supports ADME (Absorption, Distribution, Metabolism, Excretion) studies for new drug candidates targeting epigenetic regulation.
• Diagnostic Assay Development: Utilized in the calibration and validation of clinical diagnostic kits for conditions related to methylation cycle disorders.
• Biochemical Research Reagent: A high-purity reagent for fundamental research in biochemistry and molecular biology concerning cellular methylation states.

Basic Information

Product Name	5''-(Methyl-D3-Thio)Adenosine
CAS No.	174838-38-1
Molecular Formula	C11H13D3N5O4S
Molecular Weight	305.33 g/mol
Synonyms	S-Adenosyl-L-methionine (methyl-d3); SAMe (methyl-d3); AdoMet (methyl-d3); 5'-Deoxy-5'-(methyl-d3-thio)adenosine; (2S)-2-Amino-4-[[(2S,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-dihydroxyoxolan-2-yl]methyl-(methyl-d3)sulfonio]butanoate; L-S-Adenosylmethionine (methyl-d3); Isotopically labeled S-adenosylmethionine
EINECS	Contact for details

Quality Control

Our 5''-(Methyl-D3-Thio)Adenosine is manufactured under strict quality protocols to ensure high chemical and isotopic purity, critical for sensitive analytical applications. Each batch is supported by a comprehensive Certificate of Analysis (COA) detailing purity, isotopic enrichment (D3), and impurity profiles. We adhere to relevant standards for high-purity biochemical reagents, ensuring reliability and consistency for your research.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95%
Isotopic Enrichment (D3)	≥ 98 atom % D
Water Content (KF)	≤ 2.0 %
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.