Description

N4-Benzoyl-2'-Deoxy-5'-o-Dmt-2'-Fluoro-Cytidine Phosphoramidite is a high-purity, protected nucleoside phosphoramidite monomer essential for the solid-phase synthesis of oligonucleotides. This compound is critical for ensuring high coupling efficiency and sequence fidelity in the automated production of modified DNA and RNA strands. It is primarily used by research institutions, diagnostic developers, and pharmaceutical companies engaged in the development of antisense oligonucleotides, siRNAs, aptamers, and other advanced therapeutic nucleic acids.

Application

• Oligonucleotide Synthesis: As a key building block (monomer) in automated solid-phase synthesizers for the introduction of 2'-fluoro-cytidine modifications into DNA/RNA sequences.
• Therapeutic Nucleic Acid R&D: For the research and development of antisense oligonucleotides (ASOs), small interfering RNAs (siRNAs), and aptamers with enhanced nuclease resistance and binding affinity.
• Diagnostic Probe Manufacture: In the production of labeled probes and primers for molecular diagnostics, including PCR, FISH, and microarray technologies.
• Structural Biology Studies: To synthesize modified oligonucleotides for investigating RNA structure, function, and protein-nucleic acid interactions.
• Pharmaceutical Development: For the GMP-compliant synthesis of active pharmaceutical ingredients (APIs) in oligonucleotide-based drugs.
• Bioconjugation & Labeling: Serves as a precursor for creating functionalized oligonucleotides with specific tags or linkers for downstream applications.

Basic Information

Product Name	N4-Benzoyl-2'-Deoxy-5'-o-Dmt-2'-Fluoro-Cytidine Phosphoramidite
CAS No.	161442-19-9
Molecular Formula	C47H52FN4O8P
Molecular Weight	858.92 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-2'-deoxy-2'-fluoro-N4-benzoylcytidine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; 2'-F-dC(Bz) Phosphoramidite; 2'-Fluoro-2'-deoxycytidine phosphoramidite, N4-Benzoyl-5'-O-DMT protected; 2'-F-dC Phosphoramidite; 5'-O-DMT-2'-fluoro-2'-deoxycytidine, N4-benzoyl-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; 2'-Fluoro Cytidine Phosphoramidite; CAS 161442-19-9
EINECS	Contact for details

Quality Control

Every batch of our N4-Benzoyl-2'-Deoxy-5'-o-Dmt-2'-Fluoro-Cytidine Phosphoramidite is manufactured and tested under strict quality management systems. We ensure compliance with stringent purity standards required for oligonucleotide synthesis, with comprehensive release testing including HPLC purity, 31P NMR, and 1H NMR for structural confirmation and identity. Certificates of Analysis (COA) with detailed chromatographic data are provided for traceability and quality assurance.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C ± 5°C under an inert atmosphere (argon or nitrogen) in a desiccated environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation and moisture ingress.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (31P NMR)	Conforms to reference standard
Purity (HPLC)	≥ 98.0%
Moisture Content (KF)	≤ 1.0%
Solubility	Clear, colorless solution in anhydrous acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.