Description

Phosphoramidous Acid, N,n-Bis(1-Methylethyl)-, 13,13-Bis(4-Methoxyphenyl)-13-Phenyl-3,6,9,12-Tetraoxatridec-1-Yl 2-Cyanoethyl Ester is a specialized phosphoramidite monomer, a critical building block in modern synthetic chemistry. This high-purity compound is engineered to provide exceptional reliability and consistency in demanding applications where precise molecular assembly is paramount. It is primarily utilized by research institutions and industrial manufacturers in the fields of oligonucleotide synthesis, materials science, and advanced pharmaceutical development.

Application

• Solid-Phase Oligonucleotide Synthesis: Serving as a key phosphoramidite monomer for the automated synthesis of DNA and RNA sequences for research, diagnostics, and therapeutic applications.
• Antisense Oligonucleotide (ASO) & siRNA Production: A crucial raw material in the manufacture of novel therapeutic agents targeting genetic diseases.
• Probe and Primer Synthesis: Used in the production of labeled and unlabeled nucleic acid probes for genetic testing, PCR, and sequencing.
• Phosphorothioate Backbone Modification: Enables the synthesis of nuclease-resistant oligonucleotides with enhanced stability for in vivo applications.
• Materials Science & Surface Functionalization: Acts as a sophisticated linker or precursor for creating functionalized surfaces and nanomaterials.
• Process Development & Scale-Up: Supports process optimization and cGMP manufacturing for oligonucleotide-based Active Pharmaceutical Ingredients (APIs).

Basic Information

Product Name	Phosphoramidous Acid, N,n-Bis(1-Methylethyl)-, 13,13-Bis(4-Methoxyphenyl)-13-Phenyl-3,6,9,12-Tetraoxatridec-1-Yl 2-Cyanoethyl Ester
CAS No.	158041-83-9
Molecular Formula	C41H57N2O8P
Molecular Weight	736.88 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-2'-deoxyadenosine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; dA-CE Phosphoramidite (DMT-on); 2'-Deoxyadenosine, 5'-O-[bis(4-methoxyphenyl)phenylmethyl]-2'-deoxy-, 3'-[(2-cyanoethyl) N,N-diisopropylphosphoramidite]; DMT-dA(bz) Phosphoramidite; N6-Benzoyl-2'-deoxyadenosine-3'-phosphoramidite; Amidite dA(Bz); CAS 158041-83-9
EINECS	Contact for details

Quality Control

Every batch of our phosphoramidite monomers is produced and tested under strict quality management systems. We employ advanced analytical techniques including HPLC, NMR (31P & 1H), and mass spectrometry to ensure identity, purity, and performance meet the exacting standards required for oligonucleotide synthesis. A comprehensive Certificate of Analysis (COA) detailing purity, moisture content, and solvent residues is provided with each shipment. Our quality commitment supports compliance with ISO 9001 standards and the needs of cGMP manufacturing environments.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dedicated freezer. This product is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation and moisture ingress. For long-term stability, store desiccated.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (31P NMR)	Conforms to reference standard
Purity (HPLC)	≥ 98.0%
Moisture Content (Karl Fischer)	≤ 0.5%
Solvent Residues (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.