Description

2'-Ome-Ibu-G Phosphoramidite is a specialized, nucleoside phosphoramidite monomer used in the automated solid-phase synthesis of oligonucleotides. This compound is critical for introducing a modified guanosine base with a 2'-O-methyl ribose and an isobutyryl (Ibu) protecting group into synthetic RNA and DNA strands. It is an essential building block for researchers and manufacturers in the fields of therapeutic oligonucleotides, antisense technology, and molecular diagnostics. The product is supplied as a high-purity reagent to ensure optimal coupling efficiency and yield in demanding synthesis applications.

Application

• Synthesis of 2'-O-Methyl Modified Oligonucleotides: Primary building block for the solid-phase synthesis of RNA analogs with enhanced nuclease resistance and binding affinity.
• Therapeutic Oligonucleotide Production: Manufacture of antisense oligonucleotides (ASOs), siRNA, and aptamers for pharmaceutical development and clinical research.
• Diagnostic Probe Development: Creation of high-specificity probes for FISH (Fluorescence In Situ Hybridization), microarrays, and PCR-based assays.
• Basic Research Tools: Used in academic and industrial labs to study RNA structure, function, and protein-RNA interactions.
• Synthesis of Chimeric Oligonucleotides: Enables the construction of mixed DNA/2'-O-methyl RNA sequences for specialized applications.

Basic Information

Product Name	2'-Ome-Ibu-G Phosphoramidite
CAS No.	150780-67-9
Molecular Formula	C41H51N6O7P
Molecular Weight	770.87 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-2'-O-methyl-N2-isobutyrylguanosine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; 2'-O-Methyl-N2-Ibu-G Phosphoramidite; 2'-O-Me-Ibu-G CE Phosphoramidite; 2'-O-Methylguanosine(Ibu) Phosphoramidite; 2'-O-Methyl G(Ibu) Phosphoramidite; 5'-DMT-2'-O-methyl-N2-isobutyryl-Guanosine-3'-phosphoramidite; Guanosine, 5'-O-[bis(4-methoxyphenyl)phenylmethyl]-2'-O-methyl-N2-(2-methyl-1-oxopropyl)-, 3'-[2-cyanoethyl bis(1-methylethyl)phosphoramidite]
EINECS	Contact for details

Quality Control

Every batch of 2'-Ome-Ibu-G Phosphoramidite is manufactured and tested under strict quality management systems. Our products undergo rigorous analytical testing, including HPLC, 31P NMR, and 1H NMR, to ensure identity, purity, and performance meet the exacting standards required for oligonucleotide synthesis. Certificates of Analysis (COA) with detailed chromatographic data are provided to guarantee traceability and batch-to-batch consistency for your critical research and production needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a desiccated environment. This product is hygroscopic (moisture-sensitive) and should be handled under dry, anhydrous conditions to maintain stability and reactivity.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (31P NMR)	Conforms to reference standard
Purity (HPLC)	≥ 98.0%
Moisture (Karl Fischer)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.