Description

2-(2,5-Dioxo-2,5-Dihydro-1H-Pyrrol-1-Yl)-3-Methylbutanoic Acid is a specialized maleimide derivative with a branched alkyl chain, serving as a versatile building block for advanced organic synthesis. Its core value lies in its ability to act as a heterobifunctional linker, enabling the precise conjugation of biomolecules and polymers through its reactive maleimide and carboxylic acid functional groups. This compound is essential for researchers and manufacturers in the pharmaceutical, bioconjugation, and advanced materials sectors who require high-purity intermediates for developing targeted drug delivery systems, diagnostic reagents, and functional polymers.

Application

• Bioconjugation Reagent: Used for site-specific protein and antibody labeling, enabling the development of antibody-drug conjugates (ADCs) and diagnostic assays.
• Pharmaceutical Intermediate: Serves as a key synthon in the synthesis of targeted therapeutics and prodrugs.
• Polymer Functionalization: Employed to introduce reactive maleimide groups onto polymer backbones (e.g., PEG, PLGA) for subsequent coupling with thiol-containing molecules.
• Crosslinking Agent: Facilitates the formation of stable thioether bonds in hydrogel formation and biomaterial fabrication.
• Peptide Synthesis: Acts as a side-chain modifier or a terminal functional group in solid-phase peptide synthesis (SPPS).
• Surface Modification: Used to create maleimide-activated surfaces on chips, beads, or sensors for immobilizing biomolecules.
• Research & Development: A critical tool in academic and industrial R&D for exploring novel bioconjugation strategies and material science applications.

Basic Information

Product Name	2-(2,5-Dioxo-2,5-Dihydro-1H-Pyrrol-1-Yl)-3-Methylbutanoic Acid
CAS No.	148991-43-9
Molecular Formula	C9H11NO4
Molecular Weight	197.19 g/mol
Synonyms	3-Methyl-2-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)butanoic acid; 1-(1-Carboxy-2-methylpropyl)-1H-pyrrole-2,5-dione; Maleimide valeric acid derivative; N-(1-Carboxy-2-methylpropyl)maleimide; 2-(2,5-Dioxopyrrol-1-yl)-3-methylbutyric acid; 3-Methyl-2-(2,5-dioxopyrrolidin-1-yl)butanoic acid (tautomer); Bioconjugation linker CAS 148991-43-9
EINECS	Contact for details

Quality Control

Our 2-(2,5-Dioxo-2,5-Dihydro-1H-Pyrrol-1-Yl)-3-Methylbutanoic Acid is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR and IR for structural confirmation, and residual solvent analysis, to ensure it meets the high standards required for sensitive bioconjugation and pharmaceutical applications. Certificates of Analysis (COA) with detailed specifications are provided for every shipment, ensuring full traceability and compliance with relevant research and cGMP guidelines where applicable.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C for long-term stability. This product is hygroscopic (moisture-sensitive); ensure the container is sealed under inert atmosphere or with desiccant after each use to prevent degradation. Keep away from strong bases and reducing agents.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Meets ICH guidelines
Heavy Metals	< 10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.