Description

4-Hydroxy Nonenal-D3 is a deuterium-labeled analog of the endogenous lipid peroxidation product 4-Hydroxynonenal (4-HNE). This stable isotope-labeled compound is an essential tool for quantitative analytical research, providing unmatched accuracy in mass spectrometry-based detection and metabolic profiling. It is primarily utilized by researchers and quality control laboratories in the pharmaceutical, biotechnology, and academic sectors for biomarker analysis, oxidative stress studies, and method validation.

Application

• Internal Standard for LC-MS/MS: Serves as a critical quantitative reference standard for the accurate measurement of endogenous 4-HNE in biological samples (e.g., plasma, tissue, cell lysates).
• Biomarker Research: Enables precise tracking and quantification of lipid peroxidation, a key process in studies of oxidative stress, aging, and related diseases.
• Metabolic Pathway Analysis: Used in tracer studies to investigate the metabolic fate and adduct formation of 4-HNE in various biological systems.
• Method Development & Validation: Essential for developing and validating highly sensitive and specific analytical methods in clinical and preclinical research.
• Pharmaceutical R&D: Supports drug discovery efforts targeting oxidative damage, particularly in therapeutic areas like neurodegeneration, cardiovascular disease, and cancer.
• Food & Nutritional Science: Applied in research assessing lipid oxidation in food products and the impact of dietary antioxidants.

Basic Information

Product Name	4-Hydroxy Nonenal-D3
CAS No.	148706-06-3
Molecular Formula	C9H11D3O2
Molecular Weight	157.23 g/mol (for monoisotopic mass)
Synonyms	4-HNE-d3; 4-Hydroxynonenal-d3; (E)-4-Hydroxy-2-nonenal-d3; 4-Hydroxy-2-nonenal-9,9,9-d3; 4-Oxononenal-d3; Lipid Peroxidation Product-d3; HNE-d3; Deuterated 4-HNE
EINECS	Contact for details

Quality Control

Every batch of 4-Hydroxy Nonenal-D3 is manufactured and analyzed under strict quality protocols to ensure the highest standards of isotopic purity and chemical identity. Our products undergo rigorous quality testing, including NMR and mass spectrometry, to confirm deuteration level and chemical purity. A comprehensive Certificate of Analysis (COA) detailing batch-specific results for identity, purity (by HPLC), isotopic enrichment, and concentration is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at or below -20°C under an inert atmosphere (e.g., argon or nitrogen). This product is easily oxidized (store under inert atmosphere) and light-sensitive (store away from light). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	Colorless to pale yellow liquid
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Isotopic Purity (Deuteration at C-9)	≥ 98 atom % D
Concentration (Typical, in solution)	1.0 mg/mL in acetonitrile or ethanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.