Description

Citric Acid-2,2,4,4-D4 is a stable isotope-labeled analog of citric acid, where four hydrogen atoms at the 2 and 4 positions are replaced with deuterium. This compound is essential as an internal standard for precise quantitative analysis using mass spectrometry, eliminating matrix effects and ensuring analytical accuracy. It is primarily required by research laboratories, pharmaceutical developers, and analytical service providers working in metabolomics, pharmacokinetic studies, and quality control for complex biological or chemical matrices.

Application

• Internal Standard for LC-MS/MS and GC-MS: Used for the accurate quantification of endogenous citric acid and related metabolites in biological fluids (plasma, urine, tissue homogenates).
• Metabolic Pathway Tracing: Employed in isotope dilution studies to investigate flux through the tricarboxylic acid (TCA) cycle and other metabolic networks.
• Pharmaceutical Drug Metabolism and Pharmacokinetics (DMPK): Critical for determining the concentration of citric acid or citrate-containing compounds in preclinical and clinical studies.
• Food and Beverage Analysis: Ensures precise measurement of citric acid content and authenticity testing in complex food matrices.
• Diagnostic Kit Manufacturing: Incorporated as a calibrated reference in clinical diagnostic assays for metabolic disorders.
• Environmental and Industrial Process Monitoring: Used to trace and quantify citrate species in environmental samples or fermentation processes.

Basic Information

Product Name	Citric Acid-2,2,4,4-D4
CAS No.	147664-83-3
Molecular Formula	C6H4D4O7
Molecular Weight	196.13 g/mol
Synonyms	(2,2,4,4-2H4)-Citric Acid; Deuterated Citric Acid (d4); Citric Acid-d4; 1,2,3-Propanetricarboxylic acid, 2-hydroxy-2,2,4,4-d4-; Citric Acid (2,2,4,4-D4); [2,2,4,4-2H]Citric Acid; Isotopic Citric Acid; TCA Cycle Metabolite Standard-d4
EINECS	Contact for details

Quality Control

Our stable isotope-labeled products are manufactured under controlled conditions and undergo rigorous analytical verification. Each batch is characterized to ensure high chemical and isotopic purity, critical for reliable analytical results. Standard testing includes NMR and mass spectrometry for isotopic enrichment verification and HPLC for chemical purity. Certificates of Analysis (COA) detailing batch-specific data, including deuterium incorporation level and purity, are provided.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C). This material is hygroscopic (moisture-sensitive). For long-term storage, consider desiccant use and storage under inert atmosphere to maintain stability and isotopic integrity.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (MS)	≥ 98 atom % D
Deuterium Incorporation	≥ 99% at positions 2,2,4,4
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.