Description

6-FAM Amidite is a critical phosphoramidite monomer used for the solid-phase synthesis of fluorescently labeled oligonucleotides. Its primary value lies in enabling the precise, site-specific incorporation of the 6-carboxyfluorescein (6-FAM) fluorophore, a widely used green-emitting dye, into DNA or RNA sequences. This product is essential for researchers and manufacturers in the fields of molecular diagnostics, genomics, and fluorescence-based assays who require high-purity, reliable labeling reagents. Consistent quality and stability are paramount for ensuring reproducible results in sensitive applications such as qPCR, sequencing, and microarray fabrication.

Application

• Oligonucleotide Labeling: Primary reagent for the automated synthesis of 5'- or internal 6-FAM-labeled DNA/RNA probes.
• Molecular Diagnostics: Manufacturing of fluorescent probes for real-time PCR (qPCR), FISH (Fluorescence In Situ Hybridization), and other diagnostic assays.
• Genomic Research: Synthesis of labeled primers and probes for sequencing, genotyping, and gene expression analysis.
• Microarray Production: Fabrication of fluorescently tagged oligonucleotides spotted onto gene chips and other array platforms.
• FRET Studies: Construction of oligonucleotides for Fluorescence Resonance Energy Transfer (FRET) based sensors and molecular beacons.
• Next-Generation Sequencing (NGS): Preparation of fluorescent adapters or custom sequencing primers.

Basic Information

Product Name	6-FAM Amidite
CAS No.	147566-42-5
Molecular Formula	C46H49N4O9P
Molecular Weight	832.87 g/mol
Synonyms	6-Carboxyfluorescein Phosphoramidite; 6-FAM Phosphoramidite; 5'-Dimethoxytrityl-6-carboxyfluorescein-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; FAM Amidite; Fluorescein Amidite; 6-FAM-CE Phosphoramidite; 6-FAM-CPG (when on solid support); 6-Fluorescein Phosphoramidite
EINECS	Contact for details

Quality Control

Our 6-FAM Amidite is manufactured under strict quality-controlled conditions to meet the exacting standards of oligonucleotide synthesis. Each batch is subjected to comprehensive analytical testing, including HPLC for purity assessment and NMR for structural confirmation, ensuring optimal coupling efficiency and performance. Certificates of Analysis (COA) with detailed chromatographic data are provided to guarantee consistent quality and traceability for your critical applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C ± 5°C under an inert atmosphere (argon or nitrogen) in a desiccated environment. This product is hygroscopic (moisture-sensitive) and easily oxidized; prolonged exposure to air, moisture, or elevated temperatures will reduce its performance. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	Orange to red viscous oil or solid
Identification (HPLC)	Retention time corresponds to reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in anhydrous acetonitrile, dichloromethane

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.