Description

Cholest-5-En-3-Ol, 3-Cephosphoramidite is a high-purity, specialized phosphoramidite derivative of cholesterol, a critical building block in oligonucleotide synthesis and lipid nanoparticle (LNP) research. Its primary value lies in enabling the precise chemical modification and conjugation of nucleic acids, which is essential for developing advanced therapeutic and diagnostic platforms. This compound is indispensable for pharmaceutical R&D teams, biotechnology companies, and academic laboratories focused on next-generation drug delivery systems, particularly for mRNA vaccines, siRNA therapeutics, and gene editing technologies.

Application

• Oligonucleotide Synthesis: Acts as a key phosphoramidite monomer for the solid-phase synthesis of cholesterol-conjugated oligonucleotides, enhancing cellular uptake and stability.
• Lipid Nanoparticle (LNP) Formulation: Serves as a functionalized lipid component for constructing novel, targeted delivery vehicles for nucleic acid therapeutics.
• mRNA Vaccine & Therapeutic Development: Used in the research and production of lipid-based delivery systems for mRNA payloads.
• siRNA and Antisense Therapy: Facilitates the creation of cholesterol-tagged siRNA and antisense oligonucleotides to improve pharmacokinetics and tissue targeting.
• Bioconjugation & Probe Development: Enables the site-specific labeling of biomolecules with cholesterol moieties for diagnostic assays and imaging studies.
• Academic & Pharmaceutical R&D: A vital reagent for fundamental research in nucleic acid chemistry, drug delivery, and molecular biology.

Basic Information

Product Name	Cholest-5-En-3-Ol, 3-Cephosphoramidite
CAS No.	143723-63-1
Molecular Formula	C30H54NO3P
Molecular Weight	507.73 g/mol
Synonyms	Cholesterol Phosphoramidite; 3'-Cholesteryl Phosphoramidite; 3-Cholesteryloxy-2-cyanoethoxy-N,N-diisopropylaminophosphine; 3-Cholesteryl CE Phosphoramidite; 3-Cholesteryl (2-cyanoethyl) diisopropylphosphoramidite; 3-Cholesteryl Phosphoramidite, 2-cyanoethyl N,N-diisopropyl; Cholesterol 3-(2-cyanoethyl diisopropylphosphoramidite)
EINECS	Contact for details

Quality Control

Every batch of Cholest-5-En-3-Ol, 3-Cephosphoramidite is manufactured and tested under strict quality management systems to ensure the high purity and consistency required for sensitive pharmaceutical and biotech applications. Our products undergo rigorous quality testing, including HPLC, NMR, and MS analysis, to ensure compliance with stringent industry standards. A comprehensive Certificate of Analysis (COA) detailing identity, purity, and impurity profiles is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C under an inert atmosphere (argon or nitrogen). This product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to air, moisture, or light will compromise its stability and reactivity.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.