Description

n-(4-(1-Pyrenyl)Butyryl)-O(1)-(4,4'-Dimethoxytrityl)-O(2)-((Diisopropylamino)(2-Cyanoethoxy)Phosphino)-3-Amino-1,2-Propanediol is a sophisticated, high-purity phosphoramidite monomer specifically engineered for oligonucleotide synthesis. This compound is critical for introducing a pyrene fluorophore label into synthetic DNA or RNA strands, enabling advanced fluorescence-based detection and analysis. It is an essential reagent for research and development in molecular diagnostics, genomics, and biopharmaceuticals, where precise labeling and high coupling efficiency are paramount.

Application

• Fluorescent Labeling in Oligonucleotide Synthesis: Primary use as a phosphoramidite monomer for the solid-phase synthesis of pyrene-labeled DNA or RNA probes.
• Molecular Beacon and Probe Development: Fabrication of sensitive detection tools for real-time PCR (qPCR), fluorescence in situ hybridization (FISH), and microarray analysis.
• Structural Biology Studies: Incorporation into oligonucleotides to study nucleic acid structure, dynamics, and protein-nucleic acid interactions via fluorescence spectroscopy.
• Diagnostic Assay Manufacturing: Production of key components for clinical diagnostic kits that require fluorescent signal generation.
• Antisense and siRNA Research: Synthesis of labeled antisense oligonucleotides or siRNAs for tracking cellular uptake and localization.
• Academic and Industrial R&D: A vital tool in biochemistry and molecular biology laboratories for developing novel assay formats and research methodologies.

Basic Information

Item	Details
Product Name	n-(4-(1-Pyrenyl)Butyryl)-O(1)-(4,4'-Dimethoxytrityl)-O(2)-((Diisopropylamino)(2-Cyanoethoxy)Phosphino)-3-Amino-1,2-Propanediol
CAS No.	143039-35-4
Molecular Formula	C55H58N3O7P
Molecular Weight	904.05 g/mol
Synonyms	Pyrene CE Phosphoramidite; 1-Pyrenebutyryl CPG; 5'-Dimethoxytrityl-1-pyrenebutyryl-glycerol-3-cyanoethyl phosphoramidite; Pyrene C4 Phosphoramidite; DMT-Pyrene C4 Phosphoramidite; 1-Pyrenebutanoic acid, 2-[[bis(1-methylethyl)amino](2-cyanoethoxy)phosphino]oxy-1-[[(4,4'-dimethoxytriphenyl)methyl]oxy]methyl]ethyl ester; Pyrene-labeled phosphoramidite; Pyrene amidite
EINECS	Contact for details

Quality Control

Every batch of this high-value phosphoramidite is manufactured and tested under strict quality control protocols to ensure optimal performance in automated synthesizers. We guarantee high purity (>98%) as determined by advanced analytical techniques including HPLC and 31P NMR. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing purity, identity, moisture content, and coupling efficiency data to support your quality assurance processes.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dedicated freezer. This compound is hygroscopic (moisture-sensitive) and strictly light-sensitive (protect from all light exposure). Allow the sealed vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	Off-white to light yellow solid
Identification (HPLC)	Conforms to reference
Identification (NMR)	Conforms to structure (1H, 31P)
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Coupling Efficiency Test	≥ 99.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.