Description

4-Methylumbelliferyl-2-Acetamido-2-Deoxy-6-Sulphate-β-D-Glucopyranoside is a specialized fluorogenic substrate designed for the sensitive detection and quantification of specific glycosidase enzyme activity. This compound matters because it provides a reliable, high-throughput analytical tool for biochemical research and diagnostic applications. It is primarily needed by researchers and quality control laboratories in the pharmaceutical, biotechnology, and diagnostic industries for enzyme characterization, inhibitor screening, and assay development.

Application

• Enzyme Activity Assays: Fluorogenic substrate for the specific detection of sulfated glycosidases, including N-acetylglucosamine-6-sulfatase (G6S) and related enzymes.
• Diagnostic Reagent Manufacturing: Key component in the production of kits for the diagnosis of lysosomal storage disorders, such as Mucopolysaccharidosis III (Sanfilippo syndrome).
• Biochemical Research: Used in high-throughput screening (HTS) platforms to identify and characterize potential enzyme inhibitors or activators for therapeutic development.
• Quality Control in Biologics: Employed to monitor enzyme activity and purity during the production and purification of recombinant therapeutic enzymes.
• Cell Biology Studies: A tool for investigating glycosidase function and localization within cellular pathways and metabolic processes.

Basic Information

Product Name	4-Methylumbelliferyl-2-Acetamido-2-Deoxy-6-Sulphate-β-D-Glucopyranoside
CAS No.	142439-99-4
Molecular Formula	C18H21NO13S
Molecular Weight	491.42 g/mol
Synonyms	4-MU-β-GlcNAc-6S; 4-Methylumbelliferyl N-acetyl-β-D-glucosaminide-6-sulfate; 4-Methylumbelliferyl 2-acetamido-2-deoxy-6-O-sulfo-β-D-glucopyranoside; 4-MU-GlcNAc-6-sulfate; 6-Sulfo-4-methylumbelliferyl N-acetyl-β-D-glucosaminide; Fluorogenic GlcNAc-6S substrate; G6S enzyme substrate
EINECS	Contact for details

Quality Control

Our 4-Methylumbelliferyl-2-Acetamido-2-Deoxy-6-Sulphate-β-D-Glucopyranoside is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation (NMR, MS), to ensure it meets the high standards required for sensitive research and diagnostic applications. Certificates of Analysis (COA) with detailed chromatographic data are provided to guarantee reproducible performance and lot-to-lot consistency.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Fluorogenic Activity	Conforms to functional assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.