Description

(2R,3S,5R)-5-(6-Amino-9H-Purin-9-Yl)-2-((Bis(4-Methoxyphenyl)(Phenyl)Methoxy)Methyl)Tetrahydrofuran-3-Yl 2-Cyanoethyl Diisopropylphosphoramidite is a high-purity phosphoramidite monomer, a critical building block in the automated synthesis of oligonucleotides. This compound is essential for the precise and scalable production of DNA and RNA sequences, enabling advancements in genetic research, diagnostics, and therapeutic development. It is primarily utilized by biotechnology, pharmaceutical, and diagnostic companies engaged in synthetic biology, antisense therapy, and PCR probe manufacturing.

Application

• Solid-Phase Oligonucleotide Synthesis: As a key monomer for the controlled addition of adenine (dA) nucleotides in automated DNA/RNA synthesizers.
• Therapeutic Oligonucleotide Production: Manufacturing of antisense oligonucleotides (ASOs), siRNA, and aptamers for targeted drug therapies.
• Diagnostic Probe and Primer Synthesis: Production of high-fidelity probes for PCR, qPCR, FISH, and next-generation sequencing applications.
• Research-Grade Oligo Synthesis: Enabling academic and industrial research in genomics, molecular biology, and genetic engineering.
• Synthesis of Modified Oligonucleotides: Foundation for creating oligonucleotides with specific backbone or base modifications for enhanced stability and specificity.
• Scale-Up cGMP Manufacturing: Suitable for use in current Good Manufacturing Practice (cGMP) environments for clinical and commercial therapeutic oligonucleotide production.

Basic Information

Product Name	(2R,3S,5R)-5-(6-Amino-9H-Purin-9-Yl)-2-((Bis(4-Methoxyphenyl)(Phenyl)Methoxy)Methyl)Tetrahydrofuran-3-Yl 2-Cyanoethyl Diisopropylphosphoramidite
CAS No.	140613-55-4
Molecular Formula	C43H49N6O6P
Molecular Weight	776.87 g/mol
Synonyms	5'-O-DMT-2'-Deoxyadenosine-3'-O-(2-Cyanoethyl-N,N-diisopropyl)phosphoramidite; dA Phosphoramidite (DMT); 2'-Deoxyadenosine, 5'-O-[bis(4-methoxyphenyl)phenylmethyl]-2'-deoxy-N6-benzoyl-3'-O-[2-cyanoethoxy(diisopropylamino)phosphino]-; DMT-dA-CE Phosphoramidite; Deoxyadenosine Phosphoramidite; Adenine Phosphoramidite Monomer; DNA Synthesis Reagent dA; N6-Benzoyl-2'-deoxyadenosine-3'-phosphoramidite
EINECS	Contact for details

Quality Control

Our phosphoramidite monomers are produced under strict quality management systems to ensure high purity and batch-to-batch consistency, critical for successful oligonucleotide synthesis. Each lot undergoes comprehensive analysis including HPLC purity assay, 31P NMR, and 1H NMR spectroscopy to confirm identity and quality. Certificates of Analysis (COA) with detailed specifications are provided for every shipment. Production can adhere to relevant standards for research and diagnostic use.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dry, moisture-free environment. Due to its hygroscopic and moisture-sensitive nature, the material should be handled in a controlled, dry atmosphere and allowed to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (31P NMR)	Conforms to reference standard
Purity (HPLC)	≥ 98.5%
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.