Description

[2H3]-N6-Methyladenosine is a stable isotope-labeled analog of the critical RNA modification N6-methyladenosine (m6A). This deuterated compound is essential for use as an internal standard in mass spectrometry-based quantitative analysis, ensuring unparalleled accuracy and reproducibility in measurement. It is a vital tool for researchers and quality control laboratories in the fields of epigenetics, RNA biology, metabolomics, and pharmaceutical development, where precise quantification of m6A dynamics is required.

Application

• Liquid Chromatography-Mass Spectrometry (LC-MS/MS) Internal Standard: Primary use for the absolute quantification of N6-methyladenosine in complex biological samples.
• Epigenetic and Transcriptomic Research: Enables precise measurement of m6A modification levels, crucial for studying gene expression regulation.
• Biomarker Discovery and Validation: Supports the development of diagnostic assays by providing a stable reference for m6A-related biomarkers.
• Pharmaceutical R&D and Bioanalysis: Used in drug metabolism and pharmacokinetics (DMPK) studies for nucleoside-based therapeutics.
• Quality Control in Diagnostic Kit Manufacturing: Serves as a calibrant or reference material in kits designed to detect RNA modifications.
• Metabolomics and Flux Analysis: Facilitates tracing studies to understand the metabolic pathways of modified nucleosides.

Basic Information

Product Name	[2H3]-N6-Methyladenosine
CAS No.	139896-43-8
Molecular Formula	C11H13D3N5O4
Molecular Weight	281.28 g/mol
Synonyms	N6-Methyladenosine-d3; Deuterated m6A; m6A-d3; 6-Methyladenosine-2,8,5'-d3; N(6)-Methyl-2',3',5'-tri-O-acetyladenosine-d3 (precursor); 9-β-D-Ribofuranosyl-N6-methyl[2,8,5'-2H3]adenine; Stable Isotope Labeled N6-Methyladenosine
EINECS	Contact for details

Quality Control

Every batch of [2H3]-N6-Methyladenosine is manufactured and analyzed under strict quality protocols to ensure the high chemical purity and isotopic enrichment required for analytical standards. Our products undergo rigorous quality testing, including HPLC, NMR, and mass spectrometry, to ensure compliance with industry standards. Certificates of Analysis (COA) detailing purity, isotopic purity (D-enrichment), and analytical data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). For long-term storage, consider desiccant or under an inert atmosphere to maintain stability.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95%
Isotopic Purity (D-enrichment)	≥ 98 atom % D
Water Content (KF)	≤ 2.0%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.