Description

Dmt-2'Fluoro-Da(Bz) Amidite is a specialized phosphoramidite monomer used in the automated solid-phase synthesis of oligonucleotides. This compound is critical for introducing a 2'-fluoro-modified deoxyadenosine residue, which significantly enhances the nuclease resistance and binding affinity of the resulting synthetic nucleic acids. It is an essential building block for research and development in therapeutic oligonucleotides, including antisense drugs, siRNA, and aptamers, as well as for diagnostic probe development.

Application

• Therapeutic Oligonucleotide Synthesis: Key monomer for manufacturing antisense oligonucleotides (ASOs) and small interfering RNA (siRNA) with improved metabolic stability.
• Diagnostic Probe Development: Used to create high-affinity, nuclease-resistant probes for fluorescence in situ hybridization (FISH) and other molecular diagnostic assays.
• Aptamer Production: Enables the synthesis of chemically modified DNA/RNA aptamers (SELEX) for therapeutic and sensor applications.
• Basic Research: Fundamental reagent for academic and industrial research into nucleic acid chemistry, structure, and function.
• CRISPR/Cas System Enhancement: Potential use in synthesizing modified guide RNAs (gRNAs) to improve gene-editing tool performance.

Basic Information

Product Name	Dmt-2'Fluoro-Da(Bz) Amidite
CAS No.	136834-22-5
Molecular Formula	C41H47FN5O7P
Molecular Weight	779.82 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-2'-fluoro-2'-deoxyadenosine, N6-benzoyl-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; 2'-F-dA(Bz) Phosphoramidite; 2'-Fluoro-2'-deoxyadenosine, N-benzoyl-5'-O-(4,4'-dimethoxytrityl)-2'-deoxy-3'-O-[(2-cyanoethyl)-(N,N-diisopropylamino)]phosphoramidite; 2'-F-dA(Bz)-CE Phosphoramidite; 2'-Fluoro-deoxyadenosine phosphoramidite (Bz protected); DA-2'F(Bz) Amidite
EINECS	Contact for details

Quality Control

Our Dmt-2'Fluoro-Da(Bz) Amidite is manufactured under strict quality systems to ensure consistency and reliability for critical oligonucleotide synthesis. Each batch is subjected to rigorous analytical testing, including HPLC for purity and 31P/1H/19F NMR for structural confirmation and to verify the absence of isomers. A comprehensive Certificate of Analysis (COA) detailing purity, moisture content, and performance in coupling efficiency tests is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C ± 5°C under an inert atmosphere (argon or nitrogen) in a desiccated environment. This product is hygroscopic (moisture-sensitive) and easily oxidized. Allow the sealed vial to equilibrate to room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure (31P, 1H, 19F)
Purity (HPLC)	≥ 98.0%
Moisture Content (KF)	≤ 0.5%
Solubility	Clear, colorless solution in anhydrous acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.