Description

n-Boc-2-Maleimidoethylamine is a versatile heterobifunctional crosslinking reagent featuring a maleimide group for thiol conjugation and a Boc-protected amine for further functionalization. This compound is critical for the site-specific modification of peptides, proteins, and other biomolecules, enabling the creation of stable conjugates for advanced research and development. It is primarily utilized by researchers and manufacturers in the fields of bioconjugation, antibody-drug conjugate (ADC) development, and proteomics.

Application

• Bioconjugation Reagent: For the site-specific coupling of thiol-containing molecules (e.g., cysteine residues in proteins, peptides, or antibodies) to amine-containing ligands, drugs, or labels.
• Antibody-Drug Conjugate (ADC) Linker Chemistry: Serves as a key building block in the synthesis of cleavable or non-cleavable linkers for attaching cytotoxic payloads to monoclonal antibodies.
• Protein and Peptide Modification: Used to introduce functional handles (e.g., fluorophores, biotin, PEG) onto biomolecules for imaging, detection, or therapeutic purposes.
• Surface Functionalization: Immobilization of biomolecules onto solid supports (e.g., biosensor chips, nanoparticles, chromatography resins) via thiol-maleimide chemistry.
• Polymer Chemistry: Synthesis of functionalized polymers and hydrogels for biomedical applications, including drug delivery systems and tissue engineering scaffolds.
• Diagnostic Assay Development: Preparation of enzyme conjugates or labeled antigens/antibodies for immunoassays (ELISA, lateral flow tests).

Basic Information

Product Name	n-Boc-2-Maleimidoethylamine
CAS No.	134272-63-2
Molecular Formula	C13H20N2O4
Molecular Weight	268.31 g/mol
Synonyms	1-Boc-2-maleimidoethylamine; N-(tert-Butoxycarbonyl)-2-maleimidoethylamine; 2-Maleimidoethylamine, N-Boc-protected; N-Boc-maleimidoethylamine; N-(2-Aminoethyl)maleimide, N-Boc derivative; Boc-NH-(CH2)2-maleimide; tert-Butyl N-[2-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)ethyl]carbamate
EINECS	Contact for details

Quality Control

Our n-Boc-2-Maleimidoethylamine is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry, to ensure it meets the high standards required for sensitive bioconjugation applications. Certificates of Analysis (COA) with detailed chromatographic data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and performance. Allow the sealed container to reach room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Melting Point	Contact for details
Water Content (KF)	≤ 0.5%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.