Description

[5',5''-2H2]Thymidine is a deuterium-labeled isotopologue of the nucleoside thymidine, where two hydrogen atoms at the 5' and 5'' positions are replaced with deuterium (²H). This compound is essential for advanced research applications requiring stable isotope tracing and internal standards for mass spectrometry. It is primarily utilized by scientists in pharmaceutical R&D, academic biochemistry, and analytical chemistry laboratories. The product is supplied with a high level of isotopic and chemical purity to ensure reliable and reproducible experimental results.

Application

• Mass Spectrometry Internal Standard: Serves as a critical, non-radioactive internal standard for the precise quantification of endogenous thymidine and its metabolites in complex biological matrices via LC-MS/MS.
• Metabolic Pathway Tracing: Used in deuterium-labeling studies to investigate DNA synthesis, cell proliferation dynamics, and nucleotide salvage pathways in vitro and in vivo.
• Pharmacokinetic/Pharmacodynamic (PK/PD) Studies: Enables accurate tracking of drug candidates that interfere with thymidine metabolism or are incorporated into DNA.
• Biochemical Research: A key reagent for enzymatic studies involving thymidine kinase and other nucleoside-metabolizing enzymes.
• Nuclear Magnetic Resonance (NMR) Spectroscopy: Acts as a useful probe in NMR studies due to the distinct spectroscopic properties of deuterium.

Basic Information

Product Name	[5',5''-2H2]Thymidine
CAS No.	132376-92-2
Molecular Formula	C₁₀H₁₂D₂N₂O₅
Molecular Weight	244.24 g/mol
Synonyms	Thymidine-5',5''-d2; 1-(2-Deoxy-β-D-erythro-pentofuranosyl)-5-methyl-2,4(1H,3H)-pyrimidinedione-5',5''-d2; 2'-Deoxythymidine-5',5''-d2; dT-d2; Deuterated Thymidine; 5',5''-Dideuterothymidine; 1-[(2R,4S,5R)-4-Hydroxy-5-(hydroxymethyl-d2)oxolan-2-yl]-5-methylpyrimidine-2,4-dione
EINECS	Contact for details

Quality Control

Every batch of [5',5''-2H2]Thymidine is manufactured and analyzed under strict quality protocols to ensure the highest standards of isotopic enrichment and chemical purity. Our products undergo rigorous quality testing, including NMR and mass spectrometry, to confirm isotopic purity and HPLC analysis for chemical purity. Certificates of Analysis (COA) detailing batch-specific results for assay, isotopic enrichment, and impurities are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment (MS)	≥ 98 atom % D
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.