Description

4,4'-Dimethoxytrityloxy-S-(2-Thio-5-Nitropyridyl)-2-Mercaptoethane is a specialized, high-purity chemical building block primarily used in oligonucleotide synthesis. This compound matters because it serves as a critical reagent for the controlled introduction of sulfur modifications, enabling the production of advanced antisense oligonucleotides and siRNAs with enhanced stability and therapeutic potential. It is essential for pharmaceutical R&D teams, biotechnology companies, and CROs focused on developing next-generation nucleic acid-based therapeutics and diagnostics.

Application

• Phosphorothioate Oligonucleotide Synthesis: Key reagent for the site-specific incorporation of phosphorothioate linkages, a common backbone modification to improve nuclease resistance.
• Therapeutic Oligonucleotide Manufacturing: Used in the solid-phase synthesis of antisense drugs, siRNA, and aptamers for targeted therapies.
• Diagnostic Probe Development: Enables the labeling and modification of DNA/RNA probes used in molecular diagnostics and hybridization assays.
• Research & Development: A vital tool in academic and industrial labs studying nucleic acid chemistry, structure, and function.
• Scale-Up GMP Production: Suitable for use in cGMP environments for the production of clinical trial materials and commercial therapeutics.
• Synthesis of Modified Nucleosides: Acts as a protecting group and functional handle in the multi-step synthesis of complex nucleoside analogs.

Basic Information

Product Name	4,4'-Dimethoxytrityloxy-S-(2-Thio-5-Nitropyridyl)-2-Mercaptoethane
CAS No.	131384-76-4
Molecular Formula	C32H29N3O6S2
Molecular Weight	615.72 g/mol
Synonyms	DMT-SPNE; 2-[(4,4'-Dimethoxytrityloxy)ethyl]disulfanyl-5-nitropyridine; S-(2-Thio-5-nitropyridyl)-2-[(4,4'-dimethoxytrityl)thio]ethan-1-ol; 2-[(4,4'-Dimethoxytrityl)thio]ethyl 2-pyridyl disulfide 5-N-oxide; DMT-S-SPyNP; Thiolation Reagent for Oligonucleotide Synthesis; 5-Nitro-2-pyridyl 2-[(4,4'-dimethoxytrityl)thio]ethyl disulfide
EINECS	Contact for details

Quality Control

Our 4,4'-Dimethoxytrityloxy-S-(2-Thio-5-Nitropyridyl)-2-Mercaptoethane is manufactured under strict quality systems to ensure batch-to-batch consistency for sensitive oligonucleotide synthesis. Each lot undergoes rigorous analytical testing, including HPLC purity assay and identity confirmation (NMR, MS), to meet the exacting standards of pharmaceutical development. A comprehensive Certificate of Analysis (COA) detailing purity, moisture content, and related substances is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) to prevent oxidation and degradation. Allow the sealed container to equilibrate to room temperature before opening to minimize moisture condensation. Store in a cool, dry, well-ventilated area away from incompatible materials.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Solubility	Soluble in acetonitrile, DMF, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.