Description

Benzyl-N,n-Diisopropylchlorophosphoramidite is a highly reactive organophosphorus compound widely used as a key phosphitylating reagent in oligonucleotide synthesis. Its primary value lies in enabling the efficient and controlled formation of phosphite triester intermediates, which are crucial for constructing the phosphate backbone of DNA and RNA strands. This reagent is essential for manufacturers and research laboratories in the pharmaceutical, biotechnology, and diagnostic sectors, particularly those focused on antisense therapeutics, PCR primers, and genetic research.

Application

• Solid-Phase Oligonucleotide Synthesis: As a critical phosphitylating agent in automated DNA/RNA synthesizers for the production of primers, probes, and therapeutic oligonucleotides.
• Antisense and siRNA Drug Development: For the manufacture of phosphoramidite monomers used in creating novel therapeutic agents that target specific mRNA sequences.
• Research & Diagnostic Reagents: Synthesis of labeled nucleotides and custom oligonucleotides for PCR, sequencing, and hybridization assays.
• Phosphoramidite Chemistry: Serving as a versatile building block for the preparation of other specialized phosphoramidites and phosphorus-containing ligands.
• Process Development: Used in scaling up the production of oligonucleotide active pharmaceutical ingredients (APIs) under cGMP conditions.

Basic Information

Product Name	Benzyl-N,n-Diisopropylchlorophosphoramidite
CAS No.	128753-68-4
Molecular Formula	C13H21ClNOP
Molecular Weight	273.74 g/mol
Synonyms	Benzyl Diisopropylchlorophosphoramidite; Chloro(N,N-diisopropylamino)benzyloxyphosphine; Benzyl Chloro(diisopropylamino)phosphine; P-Chloro-N,N-diisopropyl-P-benzylphosphinous Amide; Phosphoramidous Chloride, Benzyl Diisopropyl-; (Benzyloxy)chloro(N,N-diisopropylamino)phosphine; Bn-Diisopropylchlorophosphoramidite
EINECS	Contact for details

Quality Control

Our Benzyl-N,n-Diisopropylchlorophosphoramidite is manufactured under strict quality-controlled conditions to ensure high purity and batch-to-batch consistency, suitable for demanding synthetic applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to industry best practices and can support requirements for GMP-grade materials for pharmaceutical applications upon request.

Storage

Preserve in a tightly closed container, protected from light. Store under an inert atmosphere (argon or nitrogen) in a cool, dry place at 2-8°C due to its moisture-sensitive and easily oxidized nature. Allow the container to reach room temperature before opening to minimize moisture ingress.

Specification

Item	Specification
Appearance	Colorless to pale yellow liquid
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 97.0%
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.